Goat NODAL Polyclonal Antibody | anti-NODAL antibody

Goat Polyclonal to Human NODAL

Cat. Num. AAA242935

• Gene Names: NODAL; HTX5
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NODAL; Polyclonal Antibody; Goat Polyclonal to Human NODAL; Anti-NODAL Antibody (Internal) IHC-plus; HTX5; Nodal homolog; Nodal; mouse; homolog; Nodal homolog (mouse); Human NODAL; anti-NODAL antibody

• Host: Goat
• Reactivity: Bat, Gibbon, Bovine, Dog, Gorilla, Horse, Human, Monkey, Pig; Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NODAL.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 347

• Applicable Applications for anti-NODAL antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide (DRSQLCRKVKFQ) from internal region of human NODAL. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Bat, Horse, Pig (100%); Mouse, Rat, Hamster (92%); Elephant (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: NODAL antibody was raised against synthetic peptide (DRSQLCRKVKFQ) from internal region of human NODAL. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bovine, Dog, Bat, Horse, Pig (100%); Mouse, Rat, Hamster (92%); Elephant (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NODAL antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-NODAL antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 222352098
• NCBI GeneID: 4838
• NCBI Accession #: NP_060525.3
• NCBI GenBank Nucleotide #: NM_018055.4
• UniProt Accession #: Q96S42; Q2M3A5; Q8N4V3
• Molecular Weight: 39,561 Da
• NCBI Official Full Name: nodal homolog
• NCBI Official Synonym Full Names: nodal growth differentiation factor
• NCBI Official Symbol: NODAL
• NCBI Official Synonym Symbols: HTX5
• NCBI Protein Information: nodal homolog
• UniProt Protein Name: Nodal homolog
• Protein Family: Nodal
• UniProt Gene Name: NODAL
• UniProt Entry Name: NODAL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Cytokine; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: morphogen activity; growth factor activity; cytokine activity; receptor agonist activity; transforming growth factor beta receptor binding

Biological Process: embryonic placenta development; mesendoderm development; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; axial mesodermal cell fate specification; embryonic pattern specification; positive regulation of activin receptor signaling pathway; floor plate morphogenesis; vasculature development; regulation of gastrulation; positive regulation of cell-cell adhesion; trophectodermal cellular morphogenesis; heart looping; cell migration involved in gastrulation; maternal placenta development; placenta development; embryonic process involved in female pregnancy; stem cell maintenance; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; liver development; formation of anatomical boundary; neural fold formation; positive regulation of angiogenesis; repression of premature neural plate formation; polarity specification of proximal/distal axis; maternal process involved in parturition; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of epithelial cell proliferation; growth

Disease: Heterotaxy, Visceral, 5, Autosomal