Human nodal homolog (mouse) ELISA Kit | NODAL elisa kit

Human Nodal homolog, NODAL ELISA Kit

Cat. Num. AAA915258

• Gene Names: NODAL; HTX5
• Reactivity: Human
• Synonyms: nodal homolog (mouse); Human Nodal homolog; NODAL ELISA Kit; Human Nodal homolog (NODAL) ELISA kit; MGC138230; nodal; NODAL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human NODAL. No significant cross-reactivity or interference between human NODAL and analogues was observed.
• Sequence Length: 347

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 78 pg/ml -5000 pg/mL
• Sensitivity: <19.5 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NODAL elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NODAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NODAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NODAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NODAL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 222352098
• NCBI GeneID: 4838
• NCBI Accession #: NP_060525.3
• NCBI GenBank Nucleotide #: NM_018055.4
• UniProt Accession #: Q96S42; Q2M3A5; Q8N4V3
• Molecular Weight: 39,561 Da
• NCBI Official Full Name: nodal homolog
• NCBI Official Synonym Full Names: nodal growth differentiation factor
• NCBI Official Symbol: NODAL
• NCBI Official Synonym Symbols: HTX5
• NCBI Protein Information: nodal homolog
• UniProt Protein Name: Nodal homolog
• Protein Family: Nodal
• UniProt Gene Name: NODAL
• UniProt Entry Name: NODAL_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]

Uniprot Description

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: morphogen activity; growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor agonist activity

Biological Process: embryonic placenta development; mesendoderm development; positive regulation of caspase activity; axial mesodermal cell fate specification; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; positive regulation of activin receptor signaling pathway; floor plate morphogenesis; vasculature development; regulation of gastrulation; positive regulation of cell-cell adhesion; trophectodermal cellular morphogenesis; heart looping; cell migration involved in gastrulation; maternal placenta development; placenta development; stem cell maintenance; embryonic process involved in female pregnancy; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; liver development; formation of anatomical boundary; positive regulation of angiogenesis; neural fold formation; repression of premature neural plate formation; maternal process involved in parturition; polarity specification of proximal/distal axis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; brain development; positive regulation of epithelial cell proliferation; growth

Disease: Heterotaxy, Visceral, 5, Autosomal