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Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,Mouse brain tissue,Mouse kidney tissue,Mouse stomach tissueAll lanes: NOB1 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 KDaObserved band size: 47 KDa)

Rabbit anti-Human, Mouse NOB1 Polyclonal Antibody | anti-NOB1 antibody

NOB1 Antibody

Gene Names
NOB1; ART-4; NOB1P; MST158; MSTP158; PSMD8BP1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
NOB1; Polyclonal Antibody; NOB1 Antibody; RNA-binding protein NOB1; Phosphorylation regulatory protein HP-10; Protein ART-4; ART4; NOB1P; PSMD8BP1; MSTP158; anti-NOB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
412
Applicable Applications for anti-NOB1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
Immunogen
Recombinant Human RNA-binding protein NOB1 protein (103-229AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-160592 / sc-160593 / sc-160594
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Hela whole cell lysate,Mouse brain tissue,Mouse kidney tissue,Mouse stomach tissueAll lanes: NOB1 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 KDaObserved band size: 47 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Hela whole cell lysate,Mouse brain tissue,Mouse kidney tissue,Mouse stomach tissueAll lanes: NOB1 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47 KDaObserved band size: 47 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7051592 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid tissue using MBS7051592 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7051592 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7051592 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of A549 cells using MBS7051592 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of A549 cells using MBS7051592 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-NOB1 antibody
May play a role in mRNA degradation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,675 Da
NCBI Official Full Name
RNA-binding protein NOB1
NCBI Official Synonym Full Names
NIN1/PSMD8 binding protein 1 homolog
NCBI Official Symbol
NOB1
NCBI Official Synonym Symbols
ART-4; NOB1P; MST158; MSTP158; PSMD8BP1
NCBI Protein Information
RNA-binding protein NOB1
UniProt Protein Name
RNA-binding protein NOB1
Protein Family
UniProt Gene Name
NOB1
UniProt Synonym Gene Names
ART4; NOB1P; PSMD8BP1
UniProt Entry Name
NOB1_HUMAN

NCBI Description

In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]).[supplied by OMIM, Nov 2010]

Uniprot Description

NOB1P: May play a role in mRNA degradation. Belongs to the NOB1 family.

Protein type: RNA processing

Chromosomal Location of Human Ortholog: 16q22.3

Cellular Component: cytosol; nucleolar preribosome, small subunit precursor; nucleoplasm

Molecular Function: endoribonuclease activity

Biological Process: cleavages during rRNA processing; maturation of SSU-rRNA; rRNA processing

Research Articles on NOB1

Similar Products

Product Notes

The NOB1 nob1 (Catalog #AAA7051592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOB1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NOB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1: 500-1: 5000 IHC: 1: 20-1: 200 IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the NOB1 nob1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NOB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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