Rabbit anti-Human, Mouse NNT Polyclonal Antibody | anti-NNT antibody

NNT Polyclonal Antibody

Cat. Num. AAA3006446

• Gene Names: NNT; GCCD4
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NNT; Polyclonal Antibody; NNT Polyclonal Antibody; NAD(P) transhydrogenase; mitochondrial; Nicotinamide nucleotide transhydrogenase; Pyridine nucleotide transhydrogenase; anti-NNT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NNT polyclonal antibody detects endogenous levels of NNT protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1086

• Applicable Applications for anti-NNT antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human NNT.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NNT polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:NIH-3T3 whole cell lysate)

Related Product Information for anti-NNT antibody

The proccess of cellular respiration is carried out by integral inner mitochondrial membrane proteins that facilitate the harnessing of energy released by the oxidation of NADH. NNT (Nicotinamide nucleotide transhydrogenase), also known as mitochondrial NAD(P) transhydrogenase or pyridine nucleotide transhydrogenase, is a 1086 amino acid multi-pass mitochondrial inner membrane protein. NNT is a homodimer with an N-terminal section belonging to the AlaDH/PNT family and a C-terminal section belonging to the PNT beta subunit family. NNT catalyzes the transfer of a hydride ion from NADH to NADP+ and functions as a mitochondrial inner membrane proton pump. Using the energy of the proton gradient created by the electron transport chain, NNT produces high concentrations of NADPH, which is used in free radical detoxification and biosynthesis.

NCBI and Uniprot Product Information

• NCBI GI #: 51338801
• NCBI GeneID: 23530
• UniProt Accession #: Q13423
• Molecular Weight: ~ 114kDa
• NCBI Official Full Name: NAD(P) transhydrogenase, mitochondrial
• NCBI Official Synonym Full Names: nicotinamide nucleotide transhydrogenase
• NCBI Official Symbol: NNT
• NCBI Official Synonym Symbols: GCCD4
• NCBI Protein Information: NAD(P) transhydrogenase, mitochondrial
• UniProt Protein Name: NAD(P) transhydrogenase, mitochondrial
• Protein Family: NAD(P) transhydrogenase
• UniProt Gene Name: NNT
• UniProt Entry Name: NNTM_HUMAN

NCBI Description

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]

Uniprot Description

NNT: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane. May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland. Defects in NNT are the cause of glucocorticoid deficiency type 4 (GCCD4). A rare, potentially lethal, autosomal recessive disorder characterized by resistance to ACTH action on the adrenal cortex, adrenal insufficiency and an inability of the adrenal cortex to produce cortisol. It usually presents in the neonatal period or in early childhood with episodes of hypoglycemia and other symptoms related to cortisol deficiency, including failure to thrive, recurrent illnesses or infections, convulsions, and shock. In a small number of patients hypoglycemia can be sufficiently severe and persistent that it leads to serious long-term neurological damage or death. The diagnosis is readily confirmed with a low plasma cortisol measurement in the presence of an elevated ACTH level, and normal aldosterone and plasma renin measurements.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Mitochondrial; EC 1.6.1.2; Membrane protein, integral; Oxidoreductase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; integral to membrane; mitochondrial respiratory chain

Molecular Function: NAD(P) transhydrogenase activity; NAD(P)+ transhydrogenase (AB-specific) activity; NADP binding; NAD binding; NAD(P)+ transhydrogenase (B-specific) activity

Biological Process: cellular metabolic process; proton transport; cell redox homeostasis; tricarboxylic acid cycle

Disease: Glucocorticoid Deficiency 4