Rabbit anti-Mouse, Rat nNOS Polyclonal Antibody | anti-NOS1 antibody

Anti-nNOS(neuronal) Antibody

Cat. Num. AAA178635

• Gene Names: NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: nNOS; Polyclonal Antibody; Anti-nNOS(neuronal) Antibody; BNOS; IHPS 1; IHPS1; N-NOS; NC-NOS; NNOS; NOS 1; NOS; NOS-I; NOS1; P29475; Nitric oxide synthase; brain; nitric oxide synthase 1 (neuronal); anti-NOS1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 1434

• Applicable Applications for anti-NOS1 antibody: Western Blot (WB)
• Application Notes: Western Blot: 0.1-0.5ug/ml
• Notes: Tested Species: In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human nNOS(neuronal) (1402-1434aa VTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of nNOS (neuronal) expression in rat brain extract (lane 1) and mouse brain extract (lane 2). nNOS (neuronal) at 160KD was detected using rabbit anti- nNOS (neuronal) Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-NOS1 antibody

Rabbit IgG polyclonal antibody for Nitric oxide synthase, brain(NOS1) detection.
Background: Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

References

1. "Entrez Gene: NOS1 Nitric oxide synthase 1 (neuronal)".
2. Shteyer, E., Edvardson, S., Wynia-Smith, S. L., Pierri, C. L., Zangen, T., Hashavya, S., Begin, M., Yaacov, B., Cinamon, Y., Koplewitz, B. Z., Vromen, A., Elpeleg, O., Smith, B. C. Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. Gastroenterology 148: 533-536, 2015.
3. Xu, W., Gorman, P., Sheer, D., Bates, G., Kishimoto, J., Lizhi, L., Emson, P. Regional localization of the gene coding for human brain nitric oxide synthase (NOS1) to 12q24.2-24.31 by fluorescent in situ hybridization. Cytogenet. Cell Genet. 64: 62-63, 1993.

NCBI and Uniprot Product Information

• NCBI GI #: 10835173
• NCBI GeneID: 4842
• NCBI Accession #: NP_000611.1
• NCBI GenBank Nucleotide #: NM_000620.4
• UniProt Accession #: P29475; O75713; E9PH30
• Molecular Weight: 164,779 Da
• NCBI Official Full Name: nitric oxide synthase, brain isoform 1
• NCBI Official Synonym Full Names: nitric oxide synthase 1
• NCBI Official Symbol: NOS1
• NCBI Official Synonym Symbols: NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
• NCBI Protein Information: nitric oxide synthase, brain
• UniProt Protein Name: Nitric oxide synthase, brain
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS1
• UniProt Synonym Gene Names: N-NOS; nNOS

NCBI Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described.

Protein type: Amino Acid Metabolism - arginine and proline; EC 1.14.13.39; Oxidoreductase

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: cytoplasm; cytoskeleton; cytosol; lipid raft; mitochondrion; perinuclear region of cytoplasm; photoreceptor inner segment; protein complex; sarcolemma; sarcoplasmic reticulum; synapse

Molecular Function: cadmium ion binding; FAD binding; FMN binding; heme binding; NADP binding; nitric-oxide synthase activity; protein binding; sodium channel regulator activity

Biological Process: arginine catabolic process; cell redox homeostasis; exogenous drug catabolic process; multicellular organismal response to stress; myoblast fusion; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of hydrolase activity; negative regulation of potassium ion transport; negative regulation of serotonin uptake; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of sodium ion transport; response to heat; response to hypoxia; striated muscle contraction

Disease: Pyloric Stenosis, Infantile Hypertrophic, 1