Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS1493718 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human NMRAL1 Polyclonal Antibody | anti-NMRAL1 antibody
NMRAL1 Antibody
Gene Names
NMRAL1; HSCARG; SDR48A1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
>95%, Protein G Purified
Synonyms
NMRAL1; Polyclonal Antibody; NMRAL1 Antibody; NmrA-like family domain-containing protein 1; HSCARG; anti-NMRAL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G Purified
Sequence Length
299
Applicable Applications for anti-NMRAL1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended dilution: WB:1:500-2000
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human NmrA-like family domain-containing protein 1 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-164609
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS1493718 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of PC3 cells with MBS1493718 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-NMRAL1 antibody
Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,344 Da
NCBI Official Full Name
nmrA-like family domain-containing protein 1
NCBI Official Synonym Full Names
NmrA-like family domain containing 1
NCBI Official Symbol
NMRAL1
NCBI Official Synonym Symbols
HSCARG; SDR48A1
NCBI Protein Information
nmrA-like family domain-containing protein 1
UniProt Protein Name
NmrA-like family domain-containing protein 1
Protein Family
UniProt Gene Name
NMRAL1
UniProt Synonym Gene Names
HSCARG
UniProt Entry Name
NMRL1_HUMAN
NCBI Description
This gene encodes an NADPH sensor protein that preferentially binds to NADPH. The encoded protein also negatively regulates the activity of NF-kappaB in a ubiquitylation-dependent manner. It plays a key role in cellular antiviral response by negatively regulating the interferon response factor 3-mediated expression of interferon beta. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2015]
Uniprot Description
NMRAL1: disulfide-linked (Probable). Belongs to the NmrA-type oxidoreductase family.
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cytoplasm; nucleus; perinuclear region of cytoplasm
Research Articles on NMRAL1
Similar Products
Product Notes
The NMRAL1 nmral1 (Catalog #AAA1493718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NMRAL1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NMRAL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the NMRAL1 nmral1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NMRAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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