Mouse NMNAT2 Monoclonal Antibody | anti-NMNAT2 antibody

NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2, NMN Adenylyltransferase 2, NaMN Adenylyltransferase 2)

Cat. Num. AAA6008256

• Gene Names: NMNAT2; PNAT2; C1orf15
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NMNAT2; Monoclonal Antibody; NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2; NMN Adenylyltransferase 2; NaMN Adenylyltransferase 2); Anti -NMNAT2 (Nicotinamide Mononucleotide Adenylyltransferase 2; anti-NMNAT2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: Nady-1
• Specificity: Recognizes human NMNAT2.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.

• Applicable Applications for anti-NMNAT2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot
• Immunogen: Recombinant human NMNAT2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis using MBS6008256 at 1:2'000 dilution.|1: Human NMNAT2 (His-tagged).|2: Human NMNAT3 (His-tagged).|3: Unrelated (His-tagged) (negative control).)

Immunohistochemistry (IHC)

(Immunohistochemical staining of NMNAT2 using MBS6008256 in human brain cortex tissue (10ug/ml).|This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.)

Related Product Information for anti-NMNAT2 antibody

NMNAT2 catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form nicotinic acid mononucleotide (NaMN) as a substrate but with lower efficiency. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans. Has been shown to correlate with Alzheimer's disease in APPswe/PS1dE9 transgenic mice and delayed wallerian degeneration in cultured superior cervical ganglia (SCGs) from morphological changes, microtubule destruction and neurofilament degradation.

Product Categories/Family for anti-NMNAT2 antibody

Antibodies; Abs to Enzymes;  Transferase

NCBI and Uniprot Product Information

• NCBI GI #: 25141325
• NCBI GeneID: 23057
• NCBI Accession #: NP_733820.1
• NCBI GenBank Nucleotide #: NM_170706.3
• UniProt Accession #: Q9BZQ4; O75067; Q5T1Q3; Q8WU99; Q96QW1
• Molecular Weight: 34,439 Da
• NCBI Official Full Name: nicotinamide mononucleotide adenylyltransferase 2 isoform 2
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 2
• NCBI Official Symbol: NMNAT2
• NCBI Official Synonym Symbols: PNAT2; C1orf15
• NCBI Protein Information: nicotinamide mononucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NaMN adenylyltransferase 2; pyridine nucleotide adenylyltransferase 2; nicotinate-nucleotide adenylyltransferase 2
• UniProt Protein Name: Nicotinamide mononucleotide adenylyltransferase 2
• UniProt Gene Name: NMNAT2
• UniProt Synonym Gene Names: C1orf15; KIAA0479; NMN adenylyltransferase 2
• UniProt Entry Name: NMNA2_HUMAN

NCBI Description

This gene product belongs to the nicotinamide mononucleotide adenylyltransferase (NMNAT) enzyme family, members of which catalyze an essential step in NAD (NADP) biosynthetic pathway. Unlike the other human family member, which is localized to the nucleus, and is ubiquitously expressed; this enzyme is cytoplasmic, and is predominantly expressed in the brain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NMNAT2: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Belongs to the eukaryotic NMN adenylyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.7.18; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.7.7.1; Transferase

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: Golgi membrane; Golgi apparatus; late endosome; synapse; trans-Golgi network

Molecular Function: nicotinamide-nucleotide adenylyltransferase activity; nicotinate-nucleotide adenylyltransferase activity; ATP binding

Biological Process: NAD biosynthetic process; vitamin metabolic process; NAD metabolic process; water-soluble vitamin metabolic process