Rabbit anti-Human NMI Polyclonal Antibody | anti-NMI antibody

NMI, NT (NMI, N-myc-interactor, N-myc and STAT interactor) (Azide free) (HRP)

Cat. Num. AAA6325855

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NMI; Polyclonal Antibody; NT (NMI; N-myc-interactor; N-myc and STAT interactor) (Azide free) (HRP); N-myc and STAT interactor; anti-NMI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NMI antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NMI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NMI.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NMI Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the NMI antibody detected the NMI protein (arrow).)

Related Product Information for anti-NMI antibody

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq].

Product Categories/Family for anti-NMI antibody

Antibodies; Transcription Factors; STAT

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Fillmore, R.A., et al. Int. J. Cancer 125(3):556-564(2009). Quaye, L., et al. Br. J. Cancer 100(6):993-1001(2009). Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). Quaye, L., et al. Clin. Cancer Res. 14(18):5833-5839(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 188219599
• NCBI GeneID: 9111
• NCBI Accession #: NP_004679.2
• NCBI GenBank Nucleotide #: NM_004688.2
• UniProt Accession #: Q13287; Q53TI8; Q9BVE5; B5BU69
• Molecular Weight: 35,057 Da
• NCBI Official Full Name: N-myc-interactor
• NCBI Official Synonym Full Names: N-myc (and STAT) interactor
• NCBI Official Symbol: NMI
• NCBI Protein Information: N-myc-interactor; N-myc interactor
• UniProt Protein Name: N-myc-interactor
• Protein Family: N-myc-interactor
• UniProt Gene Name: NMI
• UniProt Synonym Gene Names: Nmi
• UniProt Entry Name: NMI_HUMAN

NCBI Description

NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias. [provided by RefSeq, Jul 2008]

Uniprot Description

NMI: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. Belongs to the NMI family.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; inflammatory response; JAK-STAT cascade