Rabbit NME2 Polyclonal Antibody | anti-NME2 antibody

Anti-NME2 antibody

Cat. Num. AAA176059

• Gene Names: NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Immunogen affinity purified.
• Synonyms: NME2; Polyclonal Antibody; Anti-NME2 antibody; Nucleoside diphosphate kinase B(NDK B); NME/NM23 nucleoside diphosphate kinase 2; C myc purine binding transcription factor PUF antibody; C myc transcription factor antibody; C-myc purine-binding transcription factor PUF antibody; Histidine protein kinase NDKB antibody; MGC111212 antibody; MGC2212 antibody; NDK B antibody; NDKB antibody; NDKB_HUMAN antibody; NDP kinase B antibody; NDPK B antibody; NDPKB antibody; NM23 H2 antibody; nm23-H2 antibody; NM23B antibody; nme2 antibody; Non metastatic cells 2; protein(NM23B) expressed in antibody; Nucleoside diphosphate kinase B antibody; Nucleotide diphosphate kinase B antibody; PUF antibody; anti-NME2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 152

• Applicable Applications for anti-NME2 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NME2 (137-152aa EELVDYKSCAHDWVYE), different from the related mouse and rat sequences by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-NME2 antibody, MBS176059, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: Rat Skeletal Muscle Tissue LysateLane 5: PANC Cell LysateLane 6: HELA Cell LysateLane 7: SMMC Cell LysateLane 8: U87 Cell LysateLane 9: COLO320 Cell Lysate)

Immunohistochemistry (IHC)

(Anti-NME2 antibody, MBS176059, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-NME2 antibody, MBS176059, IHC(P)IHC(P): Rat Cerebellum Tissue )

Immunocytochemistry (ICC)

(Anti-NME2 antibody, MBS176059, ICCICC: HELA Cell )

Related Product Information for anti-NME2 antibody

Description: Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase B(NME2) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: NME2(NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein(NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis, Masse et al.(2002) detected high expression of mouse Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous Nme2 expression. The mouse and human NME2 genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al.(2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 127983
• NCBI GeneID: 4831
• UniProt Accession #: P22392; Q1WM23; Q6LCT6; A8MWA3
• Molecular Weight: 30,137 Da
• NCBI Official Full Name: Nucleoside diphosphate kinase B
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 2
• NCBI Official Symbol: NME2
• NCBI Official Synonym Symbols: PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2
• NCBI Protein Information: nucleoside diphosphate kinase B; HEL-S-155an; NDP kinase B; c-myc transcription factor; histidine protein kinase NDKB; nucleotide diphosphate kinase B; c-myc purine-binding transcription factor PUF; epididymis secretory sperm binding protein Li 155an; non-metastatic cells 2, protein (NM23) expressed in; non-metastatic cells 2, protein (NM23B) expressed in
• UniProt Protein Name: Nucleoside diphosphate kinase B
• UniProt Gene Name: NME2
• UniProt Synonym Gene Names: NM23B; NDK B; NDP kinase B
• UniProt Entry Name: NDKB_HUMAN

NCBI Description

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Nov 2010]

Uniprot Description

NME2: Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. Belongs to the NDK family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.13.3; EC 2.7.4.6; Nuclear receptor co-regulator; Nucleotide Metabolism - purine; Kinase, other; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 17q21.3

Cellular Component: ruffle; centrosome; focal adhesion; mitochondrion; membrane; perinuclear region of cytoplasm; lamellipodium; cytoplasm; intermediate filament; nucleus; cytosol

Molecular Function: protein binding; gamma-tubulin binding; metal ion binding; protein histidine kinase activity; intermediate filament binding; nucleoside diphosphate kinase activity; transcription factor activity; protein kinase binding; ATP binding; single-stranded DNA binding

Biological Process: GTP biosynthetic process; integrin-mediated signaling pathway; peptidyl-histidine phosphorylation; response to cAMP; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; UTP biosynthetic process; negative regulation of myeloid leukocyte differentiation; hippocampus development; nucleoside diphosphate phosphorylation; response to testosterone stimulus; CTP biosynthetic process; regulation of transcription, DNA-dependent; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; cell adhesion; positive regulation of epithelial cell proliferation; regulation of epidermis development; negative regulation of apoptosis; purine nucleotide metabolic process; positive regulation of keratinocyte differentiation; response to amine stimulus