NMDA NR2A Subunit (100 ul) Polyclonal Antibody | anti-GRIN2A antibody

Anti-NMDA Receptor, NR2A Subunit

Cat. Num. AAA502176

• Gene Names: Grin2a; NR2A; GluN2A; NMDAR2A
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Dot Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled)
• Synonyms: NMDA NR2A Subunit (100 ul); Polyclonal Antibody; Anti-NMDA Receptor; NR2A Subunit; anti-GRIN2A antibody

• Clonality: Polyclonal
• Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 1464

• Applicable Applications for anti-GRIN2A antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blots (DB)
• Application Notes: Quality Control: Western blots performed on each lot.; ; WB: 1:1000; IHC: 1:1000; IP: 6 ul per 200 ug lysate
• Preparation and Storage: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots be taken withput freeze/thawing due to the presence of 50% glycerol. Solutions are stable for a minimum of up to 1 year at -20°C.

Related Product Information for anti-GRIN2A antibody

Affinity purified rabbit polyclonal antibody

References

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.
• Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.

NCBI and Uniprot Product Information

• NCBI GI #: 140971205
• NCBI GeneID: 24409
• NCBI Accession #: NP_036705.3
• NCBI GenBank Nucleotide #: NM_012573.3
• UniProt Accession #: Q00959; O08948; Q63728
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2A
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• NCBI Official Symbol: Grin2a
• NCBI Official Synonym Symbols: NR2A; GluN2A; NMDAR2A
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2A
• Protein Family: Glutamate receptor ionotropic
• UniProt Gene Name: Grin2a
• UniProt Synonym Gene Names: GluN2A; NMDAR2A; NR2A
• UniProt Entry Name: NMDE1_RAT

NCBI Description

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

Uniprot Description

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with AIP1. Found in a complex with GRIN1 and GRIN3B. Interacts with HIP1 and NETO1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with LRFN2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Ref.8.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]