Immunohistochemistry (IHC)
(Immunochemical staining of human NLRC4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human NLRC4 Polyclonal Antibody | anti-NLRC4 antibody
Anti-NLRC4 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
NLRC4; Polyclonal Antibody; Anti-NLRC4 Antibody; Rabbit Polyclonal; NLRC4 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CARD12 Antibody; Anti-CLAN Antibody; Anti-CLAN1 Antibody; Anti-CLANA Antibody; Anti-CLANB Antibody; Anti-CLANC Antibody; Anti-CLAND Antibody; Anti-CLR2.1 Antibody; Anti-IPAF Antibody; Anti-UNQ6189/PRO20215 Antibody; anti-NLRC4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NLRC4
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NLRC4 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human NLRC4 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NLRC4 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human NLRC4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NLRC4 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-NLRC4 antibody
Inflammasome activation is critical for host defenses against various microbial infections. Activation of the NLRC4 inflammasome requires detection of flagellin or type III secretion system (T3SS) components by NLR family apoptosis inhibitory proteins (NAIPs). NLRC4 is a cytosolic member of the NOD-like receptor family that is expressed in innate immune cells. It senses indirectly bacterial flagellin and type III secretion systems, and responds by assembling an inflammasome complex that promotes caspase-1 activation and pyroptosis. NLRC4 and NLRP3, of the NOD-like receptor (NLR) family of intracellular proteins, are expressed in innate immune cells and are thought to nucleate distinct inflammasome complexes that promote caspase-1 activation, secretion of the proinflammatory cytokines IL-1beta and IL-18, and a form of cell death termed pyroptosis.
References
Zhao Y, et al. (2011) The nlrc4 inflammasome receptors for bacterial flagellin and type iii secretion apparatus. Nature 477 (7366): 596-600.Zhang L, et al. (2015) Cryo-em structure of the activated naip2-nlrc4 inflammasome reveals nucleated polymerization. Science 350 (6259): 404-409.Qu Y, et al. (2012) Phosphorylation of nlrc4 is critical for inflammasome activation. Nature 490 (7421): 539-542.
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Product Notes
The NLRC4 (Catalog #AAA8109805) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NLRC4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLRC4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the NLRC4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLRC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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