Rabbit NLE1 Polyclonal Antibody | anti-NLE1 antibody

NLE1 (Y333) Polyclonal Antibody

Cat. Num. AAA3003665

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NLE1; Polyclonal Antibody; NLE1 (Y333) Polyclonal Antibody; Notchless protein homolog 1; HUSSY-07; anti-NLE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NLE1 (Y333) polyclonal antibody detects endogenous levels of NLE1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 193

• Applicable Applications for anti-NLE1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 302-350 of Human NLE1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NLE1 (Y333) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (20ug) Lane2:K562 whole cell lysate (20ug) Lane3:HCT116 whole cell lysate (20ug) Lane4:PC12 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug))

Related Product Information for anti-NLE1 antibody

The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication. Notch receptors play an important role in development and cell-fate decisions. Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1). NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice. Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development. Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation. It may also play a role in 60S ribosomal subunit biogenesis in yeast. NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.

NCBI and Uniprot Product Information

• NCBI GI #: 62234438
• NCBI Accession #: NP_001014445.1
• NCBI GenBank Nucleotide #: NP_001014445.1
• UniProt Accession #: Q9NVX2
• Molecular Weight: ~ 48kDa
• NCBI Official Full Name: notchless protein homolog 1 isoform b
• UniProt Protein Name: Notchless protein homolog 1
• Protein Family: Notchless protein
• UniProt Gene Name: NLE1
• UniProt Entry Name: NLE1_HUMAN

Uniprot Description

NLE1: Belongs to the NLE1/RSA4 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nucleolus; nucleus

Biological Process: Notch signaling pathway; inner cell mass cell differentiation; negative regulation of mitotic cell cycle; ribosomal large subunit biogenesis and assembly