Rabbit PNN Polyclonal Antibody | anti-PNN antibody

PNN Polyclonal Antibody

Cat. Num. AAA3007574

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PNN; Polyclonal Antibody; PNN Polyclonal Antibody; Pinin; 140 kDa nuclear and cell adhesion-related phosphoprotein; Desmosome-associated protein; Domain-rich serine protein; DRS protein; DRSP; Melanoma metastasis clone A protein; Nuclear protein SDK3; SR-like protein; DRS; MEMA; anti-PNN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PNN polyclonal antibody detects endogenous levels of PNN protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 717

• Applicable Applications for anti-PNN antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human PNN.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PNN polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug))

Related Product Information for anti-PNN antibody

Pinin is a desmosomal associated protein involved with the maintenance of cell to cell adhesion of the epithelium. Pinin is a widespread protein and has been shown to play an important role in cell adhesion through its interaction with nuclear complexes. Pinin is also involved in pre-mRNA splicing through its interactions with a C-terminal RS domain of Cyclophilin G, a MOCA-type nuclear cyclophilin. Pinin becomes nuclear during the early stages of embryonic development and remains so throughout the entire period. Defects or lack of Pinin can be lethal at perinatal stages and causes defects in the cardiac outflow tract, axial skeleton, palate and dorsal dermis.

NCBI and Uniprot Product Information

• NCBI GI #: 33356174
• NCBI Accession #: NP_002678.2
• NCBI GenBank Nucleotide #: NP_002678.2
• UniProt Accession #: Q9H307
• Molecular Weight: ~ 140kDa
• NCBI Official Full Name: pinin
• UniProt Protein Name: Pinin
• Protein Family: Pinin
• UniProt Gene Name: PNN
• UniProt Synonym Gene Names: DRS; MEMA; DRS protein; DRSP
• UniProt Entry Name: PININ_HUMAN

Uniprot Description

PNN: Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma. Belongs to the pinin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Spliceosome; RNA-binding; RNA splicing; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q21.1

Cellular Component: nucleoplasm; desmosome; membrane; cytoplasm; plasma membrane; intermediate filament; nuclear speck; intercellular junction

Molecular Function: DNA binding; structural molecule activity

Biological Process: nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cell adhesion