Rabbit Nkx-3.1 Polyclonal Antibody | anti-Nkx-3.1 antibody

Nkx-3.1 (L2) Polyclonal Antibody

Cat. Num. AAA3001109

• Gene Names: NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Nkx-3.1; Polyclonal Antibody; Nkx-3.1 (L2) Polyclonal Antibody; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3-1; NKX3.1; NKX3A; anti-Nkx-3.1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NKX3.1 (L2) polyclonal antibody detects endogenous levels of NKX3.1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 159

• Applicable Applications for anti-Nkx-3.1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminual of Human Nkx-3.1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Nkx-3. 1 (L2) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-Nkx-3.1 antibody

The homeobox gene Nkx-3.1 is the human homolog of Drosophila bagpipe, which, in conjunction with tinman, determines cell fate in the dorsal mesoderm. In mammalian species, Nkx-3.1 is predominantly expressed in prostate, and it regulates prostate development in response to sonic hedgehog (Shh) signaling by exerting growth-suppressive and differentiating effects on prostatic epithelium. Nkx-3.1 is also expressed at lower levels in other tissues, including the heart and gut, in a Shh independent manner, where it plays a role in regulating proliferation of glandular epithelium and in the formation of ducts in prostate and minor salivary glands. Nkx-3.1 preferentially binds the TAAGTA sequence, which has not been reported for any other NK class homeoprotein. The human Nkx-3.1 gene is located on chromosome 8q21, which frequently undergoes a loss of heterozygosity, and although Nkx-3.1 is not a tumor suppressor gene, it may be a useful marker for benign and malignant prostate epithelium.

NCBI and Uniprot Product Information

• NCBI GI #: 373432628
• NCBI GeneID: 4824
• NCBI Accession #: NP_001243268.1
• NCBI GenBank Nucleotide #: NP_001243268.1
• UniProt Accession #: Q99801
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: homeobox protein Nkx-3.1 isoform 2
• NCBI Official Synonym Full Names: NK3 homeobox 1
• NCBI Official Symbol: NKX3-1
• NCBI Official Synonym Symbols: NKX3; BAPX2; NKX3A; NKX3.1
• NCBI Protein Information: homeobox protein Nkx-3.1
• UniProt Protein Name: Homeobox protein Nkx-3.1
• UniProt Gene Name: NKX3-1
• UniProt Synonym Gene Names: NKX3.1; NKX3A
• UniProt Entry Name: NKX31_HUMAN

NCBI Description

This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

NKX3-1: Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Act as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells. Interacts with serum response factor (SRF). Interacts with SPDEF. Interacts with WDR77. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation. By androgens and, in the LNCAP cell line, by estrogens. Androgenic control may be lost in prostate cancer cells during tumor progression from an androgen-dependent to an androgen- independent phase. Highly expressed in the prostate and, at a lower level, in the testis. Belongs to the NK-3 homeobox family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Tumor suppressor; Transcription factor

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: intracellular; nucleus

Molecular Function: androgen receptor activity; protein binding; estrogen receptor activity; protein self-association; sequence-specific DNA binding; histone deacetylase binding; estrogen receptor binding; caspase activator activity; transcription factor binding; protein kinase activator activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; salivary gland development; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; negative regulation of insulin-like growth factor receptor signaling pathway; positive regulation of caspase activity; positive regulation of mitotic cell cycle; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; protein kinase B signaling cascade; negative regulation of mitotic cell cycle; negative regulation of epithelial cell proliferation; caspase activation; somitogenesis; pharyngeal system development; male gonad development; response to testosterone stimulus; positive regulation of phosphoinositide 3-kinase cascade; branching morphogenesis of a tube; positive regulation of protein kinase activity; positive regulation of cell division; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent; metanephros development