Rabbit anti-Human NKD2 Polyclonal Antibody | anti-NKD2 antibody

NKD2, CT (NKD2, Protein naked cuticle homolog 2) (PE)

Cat. Num. AAA6325476

• Gene Names: NKD2; Naked2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NKD2; Polyclonal Antibody; CT (NKD2; Protein naked cuticle homolog 2) (PE); Protein naked cuticle homolog 2; anti-NKD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NKD2 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-401 amino acids from the C-terminal region of human NKD2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NKD2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NKD2 antibody detected the NKD2 protein (arrow).)

Immunohistochemistry (IHC)

(NKD2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NKD2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of NKD2 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(NKD2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NKD2 antibody

In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.

Product Categories/Family for anti-NKD2 antibody

Antibodies; Wnt Proteins

References

Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010). Ding, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(36):13433-13438(2008). Li, C., et al. Mol. Biol. Cell 18(8):3081-3093(2007). Adamowicz, M., et al. Genes Chromosomes Cancer 45(9):829-838(2006). Li, C., et al. Proc. Natl. Acad. Sci. U.S.A. 101(15):5571-5576(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 14916427
• NCBI GeneID: 85409
• NCBI Accession #: NP_149111.1
• NCBI GenBank Nucleotide #: NM_033120.3
• UniProt Accession #: Q969F2; Q96EK8; Q9BSN0
• Molecular Weight: 34,314 Da
• NCBI Official Full Name: protein naked cuticle homolog 2 isoform 1
• NCBI Official Synonym Full Names: naked cuticle homolog 2
• NCBI Official Symbol: NKD2
• NCBI Official Synonym Symbols: Naked2
• NCBI Protein Information: protein naked cuticle homolog 2
• UniProt Protein Name: Protein naked cuticle homolog 2
• Protein Family: Protein naked cuticle
• UniProt Gene Name: NKD2
• UniProt Synonym Gene Names: Naked-2; hNkd2

NCBI Description

This gene encodes a member of a family of proteins that function as negative regulators of Wnt receptor signaling through interaction with Dishevelled family members. The encoded protein participates in the delivery of transforming growth factor alpha-containing vesicles to the cell membrane. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.