Human T-cell leukemia/lymphoma protein 1A ELISA Kit | TCL1A elisa kit

Human T-cell leukemia/lymphoma protein 1A ELISA Kit

Cat. Num. AAA2887157

• Gene Names: TCL1A; TCL1
• Reactivity: Human
• Synonyms: T-cell leukemia/lymphoma protein 1A; Human T-cell leukemia/lymphoma protein 1A ELISA Kit; Oncogene TCL-1; Oncogene TCL1; Protein p14 TCL1; TCL1A; TCL1; TCL1A elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human T-cell leukemia/lymphoma protein 1A
• Sequence Length: 114

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78-5000 pg/mL
• Sensitivity: < 39.3 pg/mL
• Intra-assay Precision: <=8.1%
• Inter-assay Precision: <=9.4%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for TCL1A elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148922879
• NCBI GeneID: 8115
• NCBI Accession #: NP_001092195.1
• NCBI GenBank Nucleotide #: NM_001098725.1
• UniProt Accession #: P56279; Q6IBK7
• Molecular Weight: 13,460 Da
• NCBI Official Full Name: T-cell leukemia/lymphoma protein 1A
• NCBI Official Synonym Full Names: T-cell leukemia/lymphoma 1A
• NCBI Official Symbol: TCL1A
• NCBI Official Synonym Symbols: TCL1
• NCBI Protein Information: T-cell leukemia/lymphoma protein 1A
• UniProt Protein Name: T-cell leukemia/lymphoma protein 1A
• Protein Family: T-cell leukemia/lymphoma protein
• UniProt Gene Name: TCL1A
• UniProt Synonym Gene Names: TCL1; Oncogene TCL1
• UniProt Entry Name: TCL1A_HUMAN

NCBI Description

Overexpression of the TCL1 gene in humans has been implicated in the development of mature T cell leukemia, in which chromosomal rearrangements bring the TCL1 gene in close proximity to the T-cell antigen receptor (TCR)-alpha (MIM 186880) or TCR-beta (MIM 186930) regulatory elements (summarized by Virgilio et al., 1998 [PubMed 9520462]). In normal T cells TCL1 is expressed in CD4-/CD8- cells, but not in cells at later stages of differentiation. TCL1 functions as a coactivator of the cell survival kinase AKT (MIM 164730) (Laine et al., 2000 [PubMed 10983986]).[supplied by OMIM, Jul 2010]

Uniprot Description

TCL1A: Enhances the phosphorylation and activation of AKT1, AKT2 and AKT3. Promotes nuclear translocation of AKT1. Enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. Homodimer. Interacts with AKT1, AKT2 and AKT3 (via PH domain). Interacts with PNPT1; the interaction has no effect on PNPT1 exonuclease activity. Restricted in the T-cell lineage to immature thymocytes and activated peripheral lymphocytes. Preferentially expressed early in T- and B-lymphocyte differentiation. Belongs to the TCL1 family.

Protein type: Protein kinase, regulatory subunit; Oncoprotein

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: cell cortex; endoplasmic reticulum; pronucleus

Molecular Function: protein binding

Biological Process: multicellular organismal development; stem cell maintenance