Rabbit NKD1 Polyclonal Antibody | anti-NKD1 antibody

NKD1, NT (NKD1, NKD, Protein naked cuticle homolog 1) (Azide free) (HRP)

Cat. Num. AAA6325459

• Gene Names: NKD1; Naked1
• Reactivity: Human; Predicted: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NKD1; Polyclonal Antibody; NT (NKD1; NKD; Protein naked cuticle homolog 1) (Azide free) (HRP); Protein naked cuticle homolog 1; anti-NKD1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NKD1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NKD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the N-terminal region of human NKD1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NKD1 Antibody (N-term) western blot analysis in mouse lung,heart,bladder tissue lysates (35ug/lane).This demonstrates the NKD1 antibody detected the NKD1 protein (arrow).)

Related Product Information for anti-NKD1 antibody

In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.

Product Categories/Family for anti-NKD1 antibody

Antibodies; Wnt Proteins

References

Guo, J., et al. PLoS ONE 4 (11), E7982 (2009): Creyghton, M.P., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5397-5402(2006). Creyghton, M.P., et al. Genes Dev. 19(3):376-386(2005). Yan, D., et al. Proc. Natl. Acad. Sci. U.S.A. 98(26):14973-14978(2001). Katoh, M. Int. J. Oncol. 19(5):963-969(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 14916433
• NCBI GeneID: 85407
• NCBI Accession #: NP_149110.1
• NCBI GenBank Nucleotide #: NM_033119.4
• UniProt Accession #: Q969G9; Q8WZ08; B2RC39
• Molecular Weight: 52,285 Da
• NCBI Official Full Name: protein naked cuticle homolog 1
• NCBI Official Synonym Full Names: naked cuticle homolog 1 (Drosophila)
• NCBI Official Symbol: NKD1
• NCBI Official Synonym Symbols: Naked1
• NCBI Protein Information: protein naked cuticle homolog 1; naked-1; naked cuticle-1; Dvl-binding protein
• UniProt Protein Name: Protein naked cuticle homolog 1
• Protein Family: Protein naked cuticle
• UniProt Gene Name: NKD1
• UniProt Synonym Gene Names: NKD; Naked-1; hNkd; hNkd1
• UniProt Entry Name: NKD1_HUMAN

Uniprot Description

NKD1: Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Interacts with DVL1, DVL2, DVL3 and PPP2R3A. Expression is induced by activation of the Wnt signaling pathway. Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen. Belongs to the NKD family.

Protein type: Cell development/differentiation

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: protein phosphatase type 2A complex; cytoplasm; plasma membrane

Molecular Function: protein binding; calcium ion binding; PDZ domain binding

Biological Process: Wnt receptor signaling pathway; positive regulation of protein catabolic process; eye photoreceptor cell differentiation; somatic muscle development