Rabbit anti-Rat NKCC1 Polyclonal Antibody
NKCC1 (Sodium, Potassium, Chloride Cotransporter 1, BSC2, CCC1)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
SerumSerum
Synonyms
NKCC1; Polyclonal Antibody; NKCC1 (Sodium; Potassium; Chloride Cotransporter 1; BSC2; CCC1); Anti -NKCC1 (Sodium; anti-NKCC1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat sodium, potassium, chloride cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Applicable Applications for anti-NKCC1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000ug/ml using ECL.
ELISA: 1:10,000-1:50,000 using 1ug/ml N2800A control peptide per well.
Dilution: Western Blot: 1:1000-1:5000ug/ml using ECL.
ELISA: 1:10,000-1:50,000 using 1ug/ml N2800A control peptide per well.
Immunogen
Synthetic peptide of 22aa near the C-terminus of rat NKCC1. (KLH). Cellular Localization: Cytoplasmic. Species sequence homology: mouse-95%, human-86% and bovine-82%.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-NKCC1 antibody
Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells - the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide. NKCC1/BSC2 calculated mol. wt of ~115kD; actual size is ~145kD. It is strongly expressed in the kidney and many other tissues. In mouse NKCC1 is detected in basolateral membrane of IMCD and in mesangial cells in the glomerulus and basolateral membrane of acinar cells in rat submandubular gland. In the brain, NKCC1 mRNA is most prominent in choroid plexus.
Product Categories/Family for anti-NKCC1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NKCC1
Similar Products
Product Notes
The NKCC1 (Catalog #AAA616428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKCC1 (Sodium, Potassium, Chloride Cotransporter 1, BSC2, CCC1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NKCC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1:1000-1:5000ug/ml using ECL. ELISA: 1:10,000-1:50,000 using 1ug/ml N2800A control peptide per well. Researchers should empirically determine the suitability of the NKCC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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