Rabbit anti-Human, Mouse NK3R Polyclonal Antibody | anti-TACR3 antibody

NK3R Antibody

Cat. Num. AAA150256

• Gene Names: TACR3; NKR; HH11; NK3R; NK-3R; TAC3RL
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NK3R Antibody is affinity chromatography purified via peptide column.
• Synonyms: NK3R; Polyclonal Antibody; NK3R Antibody; NKR; HH11; NK-3R; TAC3RL; TAC3R; Neuromedin-K receptor; NK-3 receptor; tachykinin receptor 3; anti-TACR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NK3R Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 465

• Applicable Applications for anti-TACR3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NK3R antibody can be used for detection of NK3R by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NK3R antibody was raised against a 18 amino acid synthetic peptide from near the center of human NK3R.
• Buffer: NK3R Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NK3R antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NK3R in RAW264.7 cell lysate with NK3R antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NK3R in human brain tissue with NK3R antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NK3R in Human Brain tissue with NK3R antibody at 20 ug/mL.)

Related Product Information for anti-TACR3 antibody

NK3R Antibody: The tachykinins are a family of small peptides that include the neurotransmitters substance P, neurokinin A, and neurokinin B, which can act on three related but distinct seven transmembrane G-proteins coupled receptors, albeit at different concentrations. The NK-3 receptor (NK3R) has greatest affinity for neurokinin B and is highly expressed in the supraoptic and paraventricular nuclei. Following binding of its ligand, NK3R activates a phosphatidylinositol-calcium second messenger system. It is likely these signals lead to the release of vasopressin and oxytocin into the circulation. NK3R may be involved in learning and memory as mice lacking this gene expressed cognitive deficits compared to normal mice. Although it has been suggested that NK3R plays a role in the regulation of vagal afferent relay neurons, it is likely that these receptors are activated by substance P or neurokinin A, as the airway nerves do not express neurokinin B. NK3R antibody does not recognize NK1R or NK2R.

NCBI and Uniprot Product Information

• NCBI GI #: 128364
• NCBI GeneID: 6870
• UniProt Accession #: P29371; Q0P510
• Molecular Weight: 52,202 Da
• NCBI Official Full Name: Neuromedin-K receptor
• NCBI Official Synonym Full Names: tachykinin receptor 3
• NCBI Official Symbol: TACR3
• NCBI Official Synonym Symbols: NKR; HH11; NK3R; NK-3R; TAC3RL
• NCBI Protein Information: neuromedin-K receptor; NK-3 receptor; neurokinin B receptor; neurokinin beta receptor
• UniProt Protein Name: Neuromedin-K receptor
• Protein Family: Neuromedin-K receptor
• UniProt Gene Name: TACR3
• UniProt Synonym Gene Names: NK3R; TAC3R; NKR; NK-3R
• UniProt Entry Name: NK3R_HUMAN

NCBI Description

This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neurokinin 3, also referred to as neurokinin B. [provided by RefSeq, Jul 2008]

Uniprot Description

TACR3: This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; tachykinin receptor activity

Biological Process: tachykinin signaling pathway; hyperosmotic salinity response; regulation of dopamine metabolic process; response to morphine; positive regulation of heart rate; response to cocaine; positive regulation of blood pressure; response to estradiol stimulus; aging

Disease: Hypogonadotropic Hypogonadism 11 With Or Without Anosmia