Western Blot (WB)
(POU5F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the POU5F2 antibody detected the POU5F2 protein (arrow).)
Rabbit anti-Human, Mouse POU5F2 Polyclonal Antibody | anti-POU5F2 antibody
POU5F2, ID (POU5F2, SPRM1, POU domain, class 5, transcription factor 2, Sperm 1 POU domain transcription factor) (APC)
Gene Names
POU5F2; SPRM-1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
POU5F2; Polyclonal Antibody; ID (POU5F2; SPRM1; POU domain; class 5; transcription factor 2; Sperm 1 POU domain transcription factor) (APC); Sperm 1 POU domain transcription factor; anti-POU5F2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-POU5F2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
POU5F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-231 amino acids from the Central region of human POU5F2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(POU5F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the POU5F2 antibody detected the POU5F2 protein (arrow).)
Western Blot (WB)
(POU5F2 Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the POU5F2 antibody detected the POU5F2 protein (arrow).)
Western Blot (WB)
(POU5F2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POU5F2 antibody detected the POU5F2 protein (arrow).)
Western Blot (WB)
(POU5F2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POU5F2 antibody detected the POU5F2 protein (arrow).)
Related Product Information for anti-POU5F2 antibody
Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell (By similarity).
Product Categories/Family for anti-POU5F2 antibody
References
Wey, E., et al. Eur. J. Biochem. 220(3):753-762(1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,051 Da
NCBI Official Full Name
POU domain, class 5, transcription factor 2
NCBI Official Synonym Full Names
POU domain class 5, transcription factor 2
NCBI Official Symbol
POU5F2
NCBI Official Synonym Symbols
SPRM-1
NCBI Protein Information
POU domain, class 5, transcription factor 2; sperm 1 POU domain transcription factor
UniProt Protein Name
POU domain, class 5, transcription factor 2
Protein Family
UniProt Gene Name
POU5F2
UniProt Synonym Gene Names
SPRM1; SPRM-1
UniProt Entry Name
PO5F2_HUMAN
Uniprot Description
POU5F2: Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell. Belongs to the POU transcription factor family. Class- 5 subfamily.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 5q15
Cellular Component: nucleus
Molecular Function: sequence-specific DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Similar Products
Product Notes
The POU5F2 pou5f2 (Catalog #AAA6336039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POU5F2, ID (POU5F2, SPRM1, POU domain, class 5, transcription factor 2, Sperm 1 POU domain transcription factor) (APC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's POU5F2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the POU5F2 pou5f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POU5F2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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