Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS647183. This demonstrates that MBS647183 detected the PREP protein (arrow).)
Rabbit anti-Human PREP Polyclonal Antibody | anti-PITRM1 antibody
PREP, ID (PREP, PEP, Prolyl endopeptidase, Post-proline cleaving enzyme)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS647183. This demonstrates that MBS647183 detected the PREP protein (arrow).)
Western Blot (WB)
(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS647183. This demonstrates that MBS647183 detected the PREP protein (arrow).)
NCBI and Uniprot Product Information
Uniprot Description
Function: ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference. Ref.6
Cofactor: Binds 1 zinc ion per subunit
By similarity.
Enzyme regulation: Inhibited by nickel and zinc excess, and slightly activated by manganese. Ref.7
Subunit structure: Homodimer
By similarity.
Subcellular location: Mitochondrion matrix Ref.6.
Tissue specificity: Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta. Ref.1
Post-translational modification: The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
Sequence similarities: Belongs to the peptidase M16 family. PreP subfamily.
Sequence caution: The sequence AAH01150.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAI39997.1 differs from that shown. Reason: Erroneous gene model prediction.
Research Articles on PITRM1
Similar Products
Product Notes
The PITRM1 pitrm1 (Catalog #AAA647183) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PREP, ID (PREP, PEP, Prolyl endopeptidase, Post-proline cleaving enzyme) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PREP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the PITRM1 pitrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PREP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
