Testing Data
(The m6A rabbit monoclonal antibody (4Âug, ) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin))
Rabbit N6-methyladenosine/m6A Monoclonal Antibody | anti-m6A antibody
N6-methyladenosine/m6A Rabbit mAb
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Dot Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
N6-methyladenosine/m6A; Monoclonal Antibody; N6-methyladenosine/m6A Rabbit mAb; N6-methyladenosine; m6A; anti-m6A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Applicable Applications for anti-m6A antibody
Nucleotide Array, Dot Blot (DB), Immunofluorescence (IF)
Application Notes
DB: 1:500-1:2000
IF: 1:50-1:200
Customer Validation: DB: Human
IF: 1:50-1:200
Customer Validation: DB: Human
Immunogen
Chemical compounds corresponding to N6-methyladenosine/m6A.
Positive Samples
Arabidopsis
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Testing Data
(The m6A rabbit monoclonal antibody (4Âug, ) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin))
Testing Data
(The m6A rabbit monoclonal antibody (4Âug, ) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)Oligomer 5 - N6-methyladenosine (AUAACUGG-m6A-CCGAAUGG-Biotin)Oligomer 6 - unmodified adenosine (AUAACUGGACCGAAUGG-Biotin))
Dot Blot (DB)
(The m6A rabbit monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.)
Dot Blot (DB)
(The m6A rabbit monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGGOligomer 9 - ATAACTGGACCGAATGGOligomer 10 - AAAAAAAAAAAAAAAA-biotin.)
Testing Data
(U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 degree C for the indicated time, and stained for m6A rabbit monoclonal antibody . DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.)
Testing Data
(U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 degree C for the indicated time, and stained for m6A rabbit monoclonal antibody . DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.)
Related Product Information for anti-m6A antibody
Background: Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466)
Product Categories/Family for anti-m6A antibody
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Product Notes
The m6A (Catalog #AAA9143553) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The N6-methyladenosine/m6A Rabbit mAb reacts with Human, Mouse, Rat, Other (Wide Range) and may cross-react with other species as described in the data sheet. AAA Biotech's N6-methyladenosine/m6A can be used in a range of immunoassay formats including, but not limited to, Nucleotide Array, Dot Blot (DB), Immunofluorescence (IF). DB: 1:500-1:2000 IF: 1:50-1:200 Customer Validation: DB: Human. Researchers should empirically determine the suitability of the m6A for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N6-methyladenosine/m6A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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