Rabbit NIK Polyclonal Antibody | anti-NIK antibody
Phospho-NIK (Thr559) Antibody
Gene Names
MAP3K14; HS; NIK; HSNIK; FTDCR1B
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
NIK; Polyclonal Antibody; Phospho-NIK (Thr559) Antibody; MAP3K14; FTDCR1B; HS; HsNIK; M3K14_HUMAN; Map3k14; Mitogen activated protein kinase kinase kinase 14; Mitogen-activated protein kinase kinase kinase 14; NF kappa beta inducing kinase; NF-kappa-beta-inducing kinase; Serine/threonine-protein kinase NIK; anti-NIK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-NIK (Thr559) Antibody detects endogenous levels of NIK only when phosphorylated at Thr559.
Tissue Specificity: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Tissue Specificity: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NIK antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human NIK around the phosphorylation site of Thr559.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.
Subunit Structure
Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100 (By similarity). Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Interacts with NLRP12; this interaction promotes proteasomal degradation of MAP3K14.
Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Subcellular Location
Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-NIK antibody
Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104,042 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 14
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 14
NCBI Official Symbol
MAP3K14
NCBI Official Synonym Symbols
HS; NIK; HSNIK; FTDCR1B
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; serine/threonine protein-kinase; serine/threonine-protein kinase NIK
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 14
Protein Family
UniProt Gene Name
MAP3K14
UniProt Synonym Gene Names
NIK; HsNIK
UniProt Entry Name
M3K14_HUMAN
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Product Notes
The NIK map3k14 (Catalog #AAA9612620) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-NIK (Thr559) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NIK can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the NIK map3k14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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