Rabbit Nicotinic Receptor, alpha 3 Polyclonal Antibody | anti-CHRNA7 antibody

Nicotinic Receptor, alpha 3

Cat. Num. AAA627615

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Highly Purified; Purified by precipitation, dialysis and chromatography.
• Synonyms: Nicotinic Receptor; alpha 3; Polyclonal Antibody; Anti -Nicotinic Receptor; anti-CHRNA7 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Nicotinic alpha3 receptor (466-474) and the reactivity of Nicotinic alpha3 receptor is ~90%. Does not react to Nicotinic alpha4 receptor, Nicotinic alpha5 receptor, Nicotinic alpha7 receptor, Nicotinic beta2 receptor, Nicotinic beta3 receptor and Nicotinic beta4 receptor. Specific for the COOH terminal of nicotinic alpha3 receptor.
• Purity/Purification: Highly Purified; Purified by precipitation, dialysis and chromatography.
• Form/Format: Supplied as a liquid in PBS, 10mg/ml BSA.

• Applicable Applications for anti-CHRNA7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunocytochemistry.; Dilution: Immunocytochemistry: 20ug/ml. Cells and tissues should be fixed using PLP fixative and ABC detection.; Western Blot: 25ug/ml. Recognizes single band at 59kD.
• Immunogen: Peptide analog of the carboxyl terminal of the nicotinic alpha3 receptor, aa466-474, attached onto a carrier protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-CHRNA7 antibody

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).

Product Categories/Family for anti-CHRNA7 antibody

Antibodies; Abs to Receptors; Cholinergic

NCBI and Uniprot Product Information

• NCBI GI #: 299116991
• NCBI GeneID: 1139
• NCBI Accession #: NP_001177384.1
• NCBI GenBank Nucleotide #: NM_001190455.2
• UniProt Accession #: P36544; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0; A8K7Q4; B4DFS0
• Molecular Weight: 56,449 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7 isoform 2
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 7 (neuronal)
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7; a7 nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha 7 neuronal nicotinic acetylcholine receptor; cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor protein, alpha-7 chain
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; integral to membrane; plasma membrane; cell junction

Molecular Function: toxin binding; protein homodimerization activity; chloride channel regulator activity; beta-amyloid binding; acetylcholine-gated cation channel activity; acetylcholine receptor activity; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity

Biological Process: cellular calcium ion homeostasis; response to nicotine; synaptic transmission; positive regulation of angiogenesis; activation of MAPK activity; calcium ion transport; positive regulation of cell proliferation; response to hypoxia; ion transport; negative regulation of tumor necrosis factor production; cognition; signal transduction; memory

Disease: Chromosome 15q13.3 Deletion Syndrome