Human Neuronal acetylcholine receptor subunit alpha-7 ELISA Kit | CHRNA7 elisa kit

Human Neuronal acetylcholine receptor subunit alpha-7 ELISA Kit

Cat. Num. AAA2887839

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Reactivity: Human
• Synonyms: Neuronal acetylcholine receptor subunit alpha-7; Human Neuronal acetylcholine receptor subunit alpha-7 ELISA Kit; CHRNA7; NACHRA7; CHRNA7 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Neuronal acetylcholine receptor subunit alpha-7. No significant cross-reactivity or interference was observed.
• Sequence Length: 502

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 0.15 ng/mL -10.0 ng/mL
• Sensitivity: 0.07 ng/mL
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNA7 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Neuronal acetylcholine receptor subunit alpha-7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal acetylcholine receptor subunit alpha-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Neuronal acetylcholine receptor subunit alpha-7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neuronal acetylcholine receptor subunit alpha-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neuronal acetylcholine receptor subunit alpha-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502831
• NCBI GeneID: 1139
• NCBI Accession #: NP_000737.1
• NCBI GenBank Nucleotide #: NM_000746.5
• Molecular Weight: 11,485 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 7 subunit
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7
• UniProt Entry Name: ACHA7_HUMAN

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

nAChRA7: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 7/CHRNA7 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, ligand-gated; Channel, cation

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; plasma membrane

Molecular Function: acetylcholine binding; acetylcholine receptor activity; acetylcholine-gated cation channel activity; beta-amyloid binding; chloride channel regulator activity; ligand-gated ion channel activity; nicotinic acetylcholine-activated cation-selective channel activity; protein homodimerization activity; toxin binding

Biological Process: activation of MAPK activity; calcium ion transport; cellular calcium ion homeostasis; cognition; negative regulation of tumor necrosis factor production; positive regulation of angiogenesis; positive regulation of cell proliferation; response to hypoxia; response to nicotine; signal transduction; synaptic transmission, cholinergic

Disease: Chromosome 15q13.3 Deletion Syndrome