Rabbit anti-Human, Mouse Nicotinamide phosphoribosyltransferase Polyclonal Antibody | anti-NAMPT antibody

Rabbit anti-human Nicotinamide phosphoribosyltransferase polyclonal Antibody(NAMPT)

Cat. Num. AAA1496755

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Nicotinamide phosphoribosyltransferase; Polyclonal Antibody; Rabbit anti-human Nicotinamide phosphoribosyltransferase polyclonal Antibody(NAMPT); NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; NAMPT; PBEF; PBEF1; anti-NAMPT antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 491

• Applicable Applications for anti-NAMPT antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-5000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Nicotinamide phosphoribosyltransferase protein (1-280aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46440 / sc-46439 / sc-67020
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Nicotinamide phosphoribosyltransferase antibody at 4ug/mlLane 1:mouse heart tissueLane 2:mouse liver tissueLane 3:mouse skeletal muscle tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 56kDaObserved band size: 56kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS1496755 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using MBS1496755 at dilution 1:100)

Related Product Information for anti-NAMPT antibody

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.

NCBI and Uniprot Product Information

• NCBI GI #: 5031977
• NCBI GeneID: 10135
• NCBI Accession #: NP_005737.1
• NCBI GenBank Nucleotide #: NM_005746.2
• UniProt Accession #: P43490; Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
• Molecular Weight: 55,521 Da
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• Protein Family: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
• UniProt Entry Name: NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Transferase; EC 2.4.2.12; Cell cycle regulation; Cytokine

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: cell junction; cytosol; extracellular space; nucleoplasm

Molecular Function: cytokine activity; drug binding; nicotinamide phosphoribosyltransferase activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; protein binding; protein homodimerization activity

Biological Process: cell-cell signaling; circadian regulation of gene expression; circadian rhythm; female pregnancy; insulin receptor signaling pathway; NAD biosynthetic process; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance; signal transduction; vitamin metabolic process; water-soluble vitamin metabolic process