Western Blot (WB)
(Western blot detection of PBEF/NAMPT in Jurkat, A549, MCF7, C6 and Hela cell lysates using PBEF/NAMPT mouse mAb (1:5000 diluted).Predicted band size:56KDa.Observed band size:52KDa.)
Mouse anti-Human, Rat PBEF/NAMPT Monoclonal Antibody | anti-PBEF/NAMPT antibody
Anti-PBEF/NAMPT Mouse mAb
Western Blot (WB)
(Western blot detection of PBEF/NAMPT in Jurkat, A549, MCF7, C6 and Hela cell lysates using PBEF/NAMPT mouse mAb (1:5000 diluted).Predicted band size:56KDa.Observed band size:52KDa.)
Western Blot (WB)
(Western blot detection of PBEF/NAMPT in Jurkat, A549, MCF7, C6 and Hela cell lysates using PBEF/NAMPT mouse mAb (1:5000 diluted).Predicted band size:56KDa.Observed band size:52KDa.)
UniPort Summary Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]
Uniprot Description
PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.
Protein type: Cell cycle regulation; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cytokine; EC 2.4.2.12; Transferase
Chromosomal Location of Human Ortholog: 7q22.3
Cellular Component: cell junction; cytosol; nucleoplasm
Molecular Function: nicotinamide phosphoribosyltransferase activity; protein binding
Biological Process: cell-cell signaling; circadian regulation of gene expression; circadian rhythm; insulin receptor signaling pathway; nicotinamide metabolic process; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on PBEF/NAMPT
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Product Notes
The PBEF/NAMPT nampt (Catalog #AAA475793) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-PBEF/NAMPT Mouse mAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PBEF/NAMPT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PBEF/NAMPT nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBEF/NAMPT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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