Western Blot (WB)
(Western blot analysis NGRN using HeLa whole cell lysates)
Rabbit anti-Human NGRN Polyclonal Antibody | anti-NGRN antibody
NGRN Antibody
Gene Names
NGRN; DSC92
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NGRN; Polyclonal Antibody; NGRN Antibody; DSC92; FI58Gm; Mesenchymal stem cell protein DSC92; Neugrin; Neugrin neurite outgrowth associated; Neurite outgrowth associated protein; Neurite outgrowth-associated protein; Ngrn; NGRN_HUMAN; Spinal cord-derived protein FI58G; anti-NGRN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NGRN antibody detects endogenous levels of total NGRN
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
291
Applicable Applications for anti-NGRN antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human NGRN
Subcellular Location
Nucleus. Secreted.
Tissue Specificity
Expressed at high levels in heart, brain and skeletal muscle. In brain, mainly expressed in neurons rather than glial cells.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis NGRN using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis NGRN using HeLa whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609997 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609997 at 1/100 staining Human gastric tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-NGRN antibody
Function: May be involved in neuronal differentiation.
Similarity: Belongs to the neugrin family.
Similarity: Belongs to the neugrin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 32 kDa
Predicted: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
neugrin
NCBI Official Synonym Full Names
neugrin, neurite outgrowth associated
NCBI Official Symbol
NGRN
NCBI Official Synonym Symbols
DSC92
NCBI Protein Information
neugrin
UniProt Protein Name
Neugrin
Protein Family
UniProt Gene Name
NGRN
Uniprot Description
Plays an essential role in mitochondrial ribosome biogenesis. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system.
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Product Notes
The NGRN ngrn (Catalog #AAA9609997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NGRN Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NGRN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the NGRN ngrn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGRN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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