Adenylate Kinase 1 Recombinant Protein | AK1 recombinant protein

Adenylate Kinase 1 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123484

• Gene Names: Ak1; Ak 1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Adenylate Kinase 1; Adenylate Kinase 1 Protein; Rat; Recombinant (His Tag); Rat AK1/Adenylate kinase 1 Protein (His Tag); adenylate kinase 1; AK1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys194

• Species: Rat
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the rat Ak1 (NP_077325.2)(Met1-Lys194) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for AK1 recombinant protein

Background: The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. Three other biomarkers (AK1, CLIC1, and SOD1) were significantly increased in both adenoma and early colorectal cancer patient plasma samples and in plasma from colorectal cancer mouse models at preclinical stages compared with controls.Which can contribute to the detection of early-stage colorectal cancer and adenomas.

References

Lu Q, et al. (1996) Adenylate kinase complements nucleoside diphosphate kinase deficiency in nucleotide metabolism. Proc Natl Acad Sci. 93 (12): 5720-5.Dzeja P, et al. (2009) Adenylate kinase and AMP signaling networks: Metabolic monitoring, signal communication and body energy sensing. Int J Mol Sci. 10 (4): 1729-72.Lagresle-Peyrou C, et al. (2008) Human adenylate kinase 2 deficiency causes a profound hematopoietic defect associated with sensorineural deafness. Nature Genetics. 41: 106 - 11.

NCBI and Uniprot Product Information

• NCBI GI #: 61889092
• NCBI GeneID: 24183
• NCBI Accession #: NP_077325.2
• NCBI GenBank Nucleotide #: NM_024349.3
• UniProt Accession #: P39069; Q5EBC5; Q9JJN3
• Molecular Weight: 21,584 Da
• NCBI Official Full Name: adenylate kinase isoenzyme 1
• NCBI Official Synonym Full Names: adenylate kinase 1
• NCBI Official Symbol: Ak1
• NCBI Official Synonym Symbols: Ak 1
• NCBI Protein Information: adenylate kinase isoenzyme 1
• UniProt Protein Name: Adenylate kinase isoenzyme 1
• Protein Family: Adenylate kinase
• UniProt Gene Name: Ak1
• UniProt Synonym Gene Names: AK 1

NCBI Description

catalyzes the conversion of ATP and AMP to ADP in adenine nucleotide metabolism [RGD, Feb 2006]

Uniprot Description

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.