Rabbit NGF Polyclonal Antibody | anti-NGF antibody

NGF Antibody

Cat. Num. AAA9402102

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: NGF; Polyclonal Antibody; NGF Antibody; Beta-NGF; HSAN5; MGC161426; MGC161428; anti-NGF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NGF protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 241

• Applicable Applications for anti-NGF antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human NGF.
• Target Name: NGF
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of mouse liver tissue, using NGF antibody.)

Related Product Information for anti-NGF antibody

Nerve growth factor (NGF) is a small, secreted protein and member of the neurotrophin family of growth factors that promote neuronal cell survival and differentiation (1). Producing cells release NGF that bind and activate TrkA high affinity receptors to mediate NGF-driven signaling. NGF also binds to a low affinity p75 (NTR) receptors, which belong to the death receptor family (2). Although NGF has been classically described as favoring neuron survival and differentiation, nerve growth factor can promote apoptosis in cells that contain p75 (NTR) and lack TrkA. NGF can induce neuron death in a variety of neurodegenerative conditions, including Alzheimer disease (3). Besides its neurotrophic actions, NGF has an effect on non-neuronal cells and may help mediate inflammation, angiogenesis, and stimulate breast cancer cell growth (4-6). NGF signaling is looking increasingly promising as potential drug targets for diseases.

Product Categories/Family for anti-NGF antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 70995319
• NCBI GeneID: 4803
• NCBI Accession #: NP_002497.2
• NCBI GenBank Nucleotide #: NM_002506.2
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF
• NCBI Protein Information: beta-nerve growth factor
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB; Beta-NGF
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity

Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; activation of MAPKK activity; nerve growth factor receptor signaling pathway; adult locomotory behavior; positive regulation of apoptosis; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; regulation of axonogenesis; sensory perception of pain; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; inflammatory response; response to electrical stimulus; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; regulation of release of sequestered calcium ion into cytosol; memory; positive regulation of nerve growth factor receptor signaling pathway; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of axonogenesis; positive regulation of transcription factor activity; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V