Rabbit Neutrophil Elastase ELISA Kit | NE elisa kit

Rabbit Neutrophil Elastase ELISA Kit

• Gene Names: ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• Reactivity: Rabbit
• Synonyms: Neutrophil Elastase; Rabbit Neutrophil Elastase ELISA Kit; NE elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of NE. No significant cross-reactivity or interference between NE and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NE and all the analogues, therefore, cross reaction may still exist in some cases.

• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for NE elisa kit

Principle of the Assay: NE ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NE antibody and an NE-HRP conjugate. The assay sample and buffer are incubated together with NE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE concentration since NE from samples and NE-HRP conjugate compete for the anti-NE antibody binding site. Since the number of sites is limited, as more sites are occupied by NE from the sample, fewer sites are left to bind NE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE concentration in each sample is interpolated from this standard curve.

Intended Uses: This NE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit NE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for NE elisa kit

Microbiology

NCBI and Uniprot Product Information

• NCBI GI #: 4503549
• NCBI GeneID: 1991
• NCBI Accession #: NP_001963.1
• NCBI GenBank Nucleotide #: NM_001972.2
• Molecular Weight: 28,518 Da
• NCBI Official Full Name: neutrophil elastase preproprotein
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: ELANE
• NCBI Official Synonym Symbols: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• NCBI Protein Information: neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
• UniProt Protein Name: Neutrophil elastase
• UniProt Gene Name: ELANE
• UniProt Synonym Gene Names: ELA2; HLE
• UniProt Entry Name: ELNE_HUMAN