Human Lin-28 Homolog A (LIN28) ELISA Kit | LIN28 elisa kit

Human Protein Lin-28 Homolog A (LIN28) ELISA Kit

Cat. Num. AAA9715475

• Gene Names: LIN28A; CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• Reactivity: Human
• Synonyms: Lin-28 Homolog A (LIN28); Human Protein Lin-28 Homolog A (LIN28) ELISA Kit; LIN28; CSDD1; FLJ12457; LIN-28; LIN28A; ZCCHC1; RNA-binding protein LIN-28; lin-28 homolog; zinc finger; CCHC domain containing 1; LIN28 elisa kit

• Reactivity: Human
• Specificity: Human Protein lin-28 homolog A (LIN28) ELISA Kit has high sensitivity and excellent specificity for detection of Human LIN28. No significant cross-reactivity or interference between Human LIN28 and analogues was observed.
• Sequence Length: 209

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Protein lin-28 homolog A (LIN28) Standard Curve.)

Related Product Information for LIN28 elisa kit

Description: This Human Protein lin-28 homolog A (LIN28) ELISA Kit employs a two-site sandwich ELISA to quantitate LIN28 in samples. An antibody specific for LIN28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIN28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIN28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIN28 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: LIN28 protein is stage-specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells; however, it is suppressed in most adult tissues except for skeletal and cardiac muscle.LIN28 mRNA is a target of the micro RNAs miR-125b and miRNA-125a.When the miREs were deleted, miRNA control over LIN28 nearly vanished. Lin28 downregulation by miR-125 involved reductions in both translational efficiency and mRNA abundance.An important target of Lin28 is Igf2, a growth and differentiation factor for muscle tissue. Interaction of Lin28 with translation initiation complexes in skeletal myoblasts and in the embryonic carcinoma cell line P19 was confirmed by localization of Lin28 to the stress granules, temporary structures that contain stalled mRNA-protein translation complexes.

NCBI and Uniprot Product Information

• NCBI GI #: 13375938
• NCBI GeneID: 79727
• NCBI Accession #: NP_078950.1
• NCBI GenBank Nucleotide #: NP_078950.1
• UniProt Accession #: Q9H9Z2
• NCBI Official Full Name: protein lin-28 homolog A
• NCBI Official Synonym Full Names: lin-28 homolog A
• NCBI Official Symbol: LIN28A
• NCBI Official Synonym Symbols: CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A
• NCBI Protein Information: protein lin-28 homolog A
• UniProt Protein Name: Protein lin-28 homolog A
• UniProt Gene Name: LIN28A
• UniProt Synonym Gene Names: CSDD1; LIN28; ZCCHC1; Lin-28A
• UniProt Entry Name: LN28A_HUMAN

NCBI Description

This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015]

Uniprot Description

LIN28A: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family.

Protein type: Translation; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: stress granule; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: mRNA binding; protein binding; miRNA binding; DNA binding; zinc ion binding; RNA binding; translation initiation factor binding

Biological Process: positive regulation of translation; regulation of transcription, DNA-dependent; pre-microRNA processing; somatic stem cell maintenance; stem cell maintenance; positive regulation of neuron differentiation; RNA 3'-end processing; germ cell development; negative regulation of glial cell differentiation