Rabbit anti-Mouse, Rat NFkB p65 Polyclonal Antibody | anti-NFkB p65 antibody

RABBIT ANTI NFkB p65

Cat. Num. AAA222359

• Gene Names: RELA; p65; NFKB3
• Reactivity: Mouse, Rat
• Applications: ELISA, Gel Super Shift Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: NFkB p65; Polyclonal Antibody; RABBIT ANTI NFkB p65; anti-NFkB p65 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Serum; Serum - liquid
• Sequence Length: 548

• Applicable Applications for anti-NFkB p65 antibody: ELISA (EIA), Gel Super Shift Assays (GS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 1/5000; Maximum Dilution: 1/25000;; Gel Super Shift Assays: Minimum Dilution: 0.5; Maximum Dilution: 1.0 ul per test;; Western Blotting: Minimum Dilution: 1/2000; Maximum Dilution: 1/5000
• Perservative Stabilisers: 0.01% Sodium Azide
• Antiserum Preparation: Antisera to NFkB p65 were raised by repeated immunisation of rabbits with highly purified antigen.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-NFkB p65 antibody

Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFkappaB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFkappaB p65 is a 551 amino acid ~65 kDa subunit of the NFkappaB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFkappaB appears to be the p65-p50 heterodimer. In the active form NFkappaB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFkappaB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IkappaB (UniProt: Q04206) In a gel supershift assay Rabbit anti Human NFkappaB p65 antibody was found to be active against all p65 containing human, mouse or rat NFkappaB complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NFkappaB p65 antibody to the NFkappaB p65 subunit can be blocked using the NFkappaB p65 control peptide (MBS237002).

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 59,910 Da
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; v-rel reticuloendotheliosis viral oncogene homolog A
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; DNA binding; transcription activator binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; defense response to virus; toll-like receptor 4 signaling pathway; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; response to insulin stimulus; toll-like receptor 10 signaling pathway; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; response to cobalamin