Western Blot (WB)
(Anti-TMED9 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:26 kDa)
Rabbit anti-Human TMED9 Polyclonal Antibody | anti-TMED9 antibody
Anti-TMED9 Antibody, Rabbit Polyclonal
Gene Names
TMED9; p25; GMP25; HSGP25L2G
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
TMED9; Polyclonal Antibody; Anti-TMED9 Antibody; Rabbit Polyclonal; TMED9/GP25L2 Antibody; Rabbit PAb; Antigen Affinity Purified; transmembrane p24 trafficking protein 9; Anti-GMP25 Antibody; Anti-GP25L2 Antibody; Anti-HSGP25L2G Antibody; Anti-p25 Antibody; anti-TMED9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMED9/GP25L2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TMED9 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 1-4uL/mg of lysate
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human TMED9/GP25L2 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human TMED9/GP25L2 (rh TMED9/GP25L2). TMED9/GP25L2 specific IgG was purified by Human TMED9/GP25L2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TMED9 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:26 kDa)
Western Blot (WB)
(Anti-TMED9 rabbit polyclonal antibody at 1:500 dilutionLane A: NCI-H1299 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:26 kDa)
Immunoprecipitation (IP)
(TMED9 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-TMED9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TMED9 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 24 kDa)
Immunoprecipitation (IP)
(TMED9 was immunoprecipitated using:Lane A:0.5 mg 293T Whole Cell Lysate4 uL anti-TMED9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TMED9 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 27 kDaObserved band size: 24 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,277 Da
NCBI Official Full Name
transmembrane emp24 domain-containing protein 9
NCBI Official Synonym Full Names
transmembrane emp24 protein transport domain containing 9
NCBI Official Symbol
TMED9
NCBI Official Synonym Symbols
p25; GMP25; HSGP25L2G
NCBI Protein Information
transmembrane emp24 domain-containing protein 9; p24alpha2; gp25L2 protein; glycoprotein 25L2; p24 family protein alpha-2
Protein Family
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Product Notes
The TMED9 (Catalog #AAA8107510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMED9 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMED9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the TMED9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMED9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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