Rabbit NFkB, p100/p52 Polyclonal Antibody

NFkB, p100/p52 (Nuclear Factor NF-kappa-B p100 Subunit, DNA-binding Factor KBF2, H2TF1, Lymphocyte Translocation Chromosome 10 Protein, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 2, Oncogene Lyt-10, Lyt10, Nuclear Factor NF-kappa-B

Cat. Num. AAA648495

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NFkB; p100/p52; Polyclonal Antibody; p100/p52 (Nuclear Factor NF-kappa-B p100 Subunit; DNA-binding Factor KBF2; H2TF1; Lymphocyte Translocation Chromosome 10 Protein; Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 2; Oncogene Lyt-10; Lyt10; Nuclear Factor NF-kappa-B; Anti -NFkB; anti-NFkB; p100/p52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human NF-kB p100/p52 around serine 865. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-NFkB, p100/p52 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.; Dilution: Immunofluorescence: 1:100-1:200; Western Blot: 1:500-1:1000; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic non-phosphopeptide corresponding to human NF-kB p100, p52 around the phosphorylation site of serine 865 (E-D-SP-A-Y).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-NFkB, p100/p52 antibody

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Product Categories/Family for anti-NFkB, p100/p52 antibody

Antibodies; Abs to Transcription Factors