Rabbit anti-Mouse, Rat NFkB, p50 Polyclonal Antibody | anti-NFKB1 antibody

NFkB, p50 (NF-kB, Nuclear Factor kappa B p50 Subunit, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1, NFkB1, DKFZp686C01211, DNA Binding Factor KBF1, EBP-1, KBF1, MGC54151, NF-kappa-B, NF-kB, Nuclear Factor kappa B DNA Binding Subuni

Cat. Num. AAA624950

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Reactivity: Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NFkB; p50; Polyclonal Antibody; p50 (NF-kB; Nuclear Factor kappa B p50 Subunit; Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells 1; NFkB1; DKFZp686C01211; DNA Binding Factor KBF1; EBP-1; KBF1; MGC54151; NF-kappa-B; NF-kB; Nuclear Factor kappa B DNA Binding Subuni; Anti -NFkB; anti-NFKB1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This immunoaffinity purified antibody recognizes a 50kD protein, corresponding to the apparent molecular masses of NF£eB, p50 on SDS-PAGE immunoblots, in samples from mouse and rat. Additional high molecular mass bands may sometimes be detected. These may respresent the p105 precursor protein. The antibody specificity is confirmed in Western blot by peptide competition studies.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in borate buffered saline, pH 8.s.

• Applicable Applications for anti-NFKB1 antibody: Western Blot (WB)
• Application Notes: Dilution: Western Blot (Colorimetric): 0.5ug/ml. Predicted MW: ~50kD and ~105kD; Immunohistochemistry: 1:50; Optimal dilutions to be determined by researcher.
• Immunogen: Synthetic peptide derived from the sequence of mouse NF-kB (coupled to KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of rat spllen tissue extract using N2302-10A.)

Related Product Information for anti-NFKB1 antibody

Nuclear factor-kappa B, also known as NFkappaB, KBF1, p110 and EBP-1, was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes (1,2). NF-kappaB is a heterodimer that consists of a 50kD DNA binding subunit (p50) and a 65 kD transactivation subunit (p65/Rel A) (3). Both of these subunits exhibit sequence homology to the protooncogene c-Rel (4). The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 (5,6) and p100 (7), respectively. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkappaB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (interleukin-1, tumor necrosis factor), and viral infection, IkappaB is phosphorylated and degraded by proteosome. The active NF-kappaB heterodimer is translocated into the nucleus and will induce gene expression.

Product Categories/Family for anti-NFKB1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 34577122
• NCBI GeneID: 4790
• NCBI Accession #: NP_003989.2
• NCBI GenBank Nucleotide #: NM_003998.3
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 105,356 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 1
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN

NCBI Description

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of cholesterol transport; negative regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to oxidative stress; negative regulation of apoptosis