Immunofluorescence (IF)
(MBS9606192 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human NFE2L3 Polyclonal Antibody | anti-NFE2L3 antibody
NFE2L3 Antibody
Gene Names
NFE2L3; NRF3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NFE2L3; Polyclonal Antibody; NFE2L3 Antibody; erythroid derived 2; like 3; NF-E2-related factor 3; NF2L3_HUMAN; NFE2-related factor 3; Nfe2l3; NRF3; Nuclear factor; Nuclear factor erythroid 2-related factor 3; anti-NFE2L3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
NFE2L3 antibody detects endogenous levels of total NFE2L3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
694
Applicable Applications for anti-NFE2L3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed in human placenta and also in B-cell and monocyte cell lines. Low expression in heart, brain, lung, skeletal muscle, kidney and pancreas.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9606192 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9606192 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using NFE2L3 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, using NFE2L3 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-NFE2L3 antibody
Function: Activates erythroid-specific, globin gene expression.
Subunit Structure: Heterodimer with MAFG, MAFK and other small MAF proteins that binds to the MAF recognition elements (MARE).
Similarity: Belongs to the bZIP family. CNC subfamily.
Subunit Structure: Heterodimer with MAFG, MAFK and other small MAF proteins that binds to the MAF recognition elements (MARE).
Similarity: Belongs to the bZIP family. CNC subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 75 kD
Predicted: 77 kDa
Predicted: 77 kDa
NCBI Official Full Name
nuclear factor erythroid 2-related factor 3
NCBI Official Synonym Full Names
nuclear factor, erythroid 2 like 3
NCBI Official Symbol
NFE2L3
NCBI Official Synonym Symbols
NRF3
NCBI Protein Information
nuclear factor erythroid 2-related factor 3
UniProt Protein Name
Nuclear factor erythroid 2-related factor 3
Protein Family
UniProt Gene Name
NFE2L3
UniProt Synonym Gene Names
NRF3; NF-E2-related factor 3; NFE2-related factor 3
NCBI Description
This gene encodes a member of the cap 'n' collar basic-region leucine zipper family of transcription factors. The encoded protein heterodimerizes with small musculoaponeurotic fibrosarcoma factors to bind antioxidant response elements in target genes. This protein is a membrane bound glycoprotein that is targeted to the endoplasmic reticulum and the nuclear envelope. Pseudogenes of this gene are found on chromosomes 16, 17, and 18. [provided by RefSeq, Mar 2009]
Uniprot Description
Activates erythroid-specific, globin gene expression.
Research Articles on NFE2L3
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Product Notes
The NFE2L3 nfe2l3 (Catalog #AAA9606192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFE2L3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFE2L3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NFE2L3 nfe2l3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFE2L3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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