Human Excitatory Amino Acid Transporter 2 (SLC1A2) ELISA Kit | EAAT2 elisa kit

Human Excitatory Amino Acid Transporter 2 (SLC1A2) ELISA Kit

Cat. Num. AAA9714284

• Gene Names: SLC1A2; HBGT; EAAT2; GLT-1; EIEE41
• Reactivity: Human
• Synonyms: Excitatory Amino Acid Transporter 2 (SLC1A2); Human Excitatory Amino Acid Transporter 2 (SLC1A2) ELISA Kit; SLC1A2; GLT-1; excitatory amino acid transporter 2; excitotoxic amino acid transporter 2; glial high affinity glutamate transporter; glutamate/aspartate transporter; sodium-dependent glutamate/asparta; EAAT2 elisa kit

• Reactivity: Human
• Specificity: Human Excitatory amino acid transporter 2 (SLC1A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC1A2. No significant cross-reactivity or interference between Human SLC1A2 and analogues was observed.
• Sequence Length: 565

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Excitatory amino acid transporter 2 (SLC1A2) Standard Curve.)

Related Product Information for EAAT2 elisa kit

Description: This Human Excitatory amino acid transporter 2 (SLC1A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC1A2 in samples. An antibody specific for SLC1A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC1A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC1A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC1A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: SLC1A2/ EAAT2 is a member of a family of the solute carrier family of proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Ceftriaxone, an antibiotic, has been shown to induce/enhance the expression of EAAT2, resulting in reduced glutamate activity. Ceftriaxone has been shown to reduce the development and expression of tolerance to opiates and other drugs of abuse. EAAT2 may possess an important role in modulating drug addiction and tolerance.

NCBI and Uniprot Product Information

• NCBI GI #: 307746865
• NCBI GeneID: 6506
• NCBI Accession #: NP_001182657.1
• NCBI GenBank Nucleotide #: NP_001182657.1
• UniProt Accession #: P43004
• NCBI Official Full Name: excitatory amino acid transporter 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 1 member 2
• NCBI Official Symbol: SLC1A2
• NCBI Official Synonym Symbols: HBGT; EAAT2; GLT-1; EIEE41
• NCBI Protein Information: excitatory amino acid transporter 2
• UniProt Protein Name: Excitatory amino acid transporter 2
• UniProt Gene Name: SLC1A2
• UniProt Synonym Gene Names: EAAT2; GLT1
• UniProt Entry Name: EAA2_HUMAN

NCBI Description

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2017]