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SDS-Page

ALDOC active protein

ALDOC, 1-364aa, Human, E Coli (Bioactivity Validated)

Gene Names
ALDOC; ALDC
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
ALDOC; 1-364aa; Human; E Coli (Bioactivity Validated); Fructose bisphosphate aldolase C; ALDC; ALDOC active protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY
Sequence Length
364
Applicable Applications for ALDOC active protein
SDS-PAGE
Antigen Species
Human
Biological Activity
Specific activity is > 6 units/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3- phosphate per minute at pH 7.5 at 37C
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for ALDOC active protein
ALDOC, also as known as fructose bisphosphate C, is a member of the class 1 fructose-biphosphate aldolase family. This protein is a ubiquitous enzyme that catalyzes the reversible aldol cleavage of fructose-bisphosphate and fructose 1-phospate to digydroxyacetone phosphate and either glyceral-dehyde-3-phosphate or glyceraldegyde, respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain. Recombinant human ALDOC was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ALDOC active protein
References
Caspi M., et al. (2014) Mol Cancer. 13:164.; Arakaki TL., et al. (2004) Protein Sci. 13 (12): 3077-84

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
230
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39.4 kDa (364aa) confirmed by MALDI-TOF
NCBI Official Full Name
fructose-bisphosphate aldolase C
NCBI Official Synonym Full Names
aldolase, fructose-bisphosphate C
NCBI Official Symbol
ALDOC
NCBI Official Synonym Symbols
ALDC
NCBI Protein Information
fructose-bisphosphate aldolase C
UniProt Protein Name
Fructose-bisphosphate aldolase C
UniProt Gene Name
ALDOC
UniProt Synonym Gene Names
ALDC

NCBI Description

This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

MiscellaneousIn vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Research Articles on ALDOC

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Product Notes

The ALDOC aldoc (Catalog #AAA206759) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ALDOC can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ALDOC aldoc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPHSYPALSA EQKKELSDIA LRIVAPGKGI LAADESVGSM AKRLSQIGVE NTEENRRLYR QVLFSADDRV KKCIGGVIFF HETLYQKDDN GVPFVRTIQD KGIVVGIKVD KGVVPLAGTD GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKISERTPS ALAILENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC PIKYTPEEIA MATVTALRRT VPPAVPGVTF LSGGQSEEEA SFNLNAINRC PLPRPWALTF SYGRALQASA LNAWRGQRDN AGAATEEFIK RAEVNGLAAQ GKYEGSGEDG GAAAQSLYIA NHAY. It is sometimes possible for the material contained within the vial of "ALDOC, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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