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Western Blot (WB)
(Western Blot: Sample: Mouse Spleen Tissue.)
Rabbit anti-Mouse Neutrophil Cytosolic Factor 4 Polyclonal Antibody | anti-NCF4 antibody
Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4)
Gene Names
Ncf4; p40phox; AI451400
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Neutrophil Cytosolic Factor 4; Polyclonal Antibody; Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4); anti-NCF4 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NCF4. It has beenselected for its ability to recognize NCF4 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AYMKNLLSLP VCVLMDPDVR IFFYQSAYDA EQVPQALRRL RPRTRKIKGV SPQGAIMDRM EAPRAEALFD FTGNSKLELS FKAGDVIFLL SKINKDWLEG TSQGATGIFP GSFVKILKDF PEDEDTTNWL RCYFYEDTGK TIKDIAVEED LSSTPLFKDL LALMRREFQR EDIALSYQDA EGDLVRLLSD EDVGLMVKQA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AYMKNLLSLP VCVLMDPDVR IFFYQSAYDA EQVPQALRRL RPRTRKIKGV SPQGAIMDRM EAPRAEALFD FTGNSKLELS FKAGDVIFLL SKINKDWLEG TSQGATGIFP GSFVKILKDF PEDEDTTNWL RCYFYEDTGK TIKDIAVEED LSSTPLFKDL LALMRREFQR EDIALSYQDA EGDLVRLLSD EDVGLMVKQA
Sequence Length
340
Applicable Applications for anti-NCF4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-5ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
NCF4 (Ala111~Pro339)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant NCF4 (Ala111~Pro339) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041108
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen Tissue.)
Western Blot (WB)
(Western Blot: Sample: Mouse Spleen Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCF4, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant NCF4, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: .Mouse Spleen Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: .Mouse Spleen Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,707 Da
NCBI Official Full Name
neutrophil cytosol factor 4
NCBI Official Synonym Full Names
neutrophil cytosolic factor 4
NCBI Official Symbol
Ncf4
NCBI Official Synonym Symbols
p40phox; AI451400
NCBI Protein Information
neutrophil cytosol factor 4
UniProt Protein Name
Neutrophil cytosol factor 4
Protein Family
UniProt Gene Name
Ncf4
UniProt Synonym Gene Names
NCF-4; p40phox
Uniprot Description
p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed.
Protein type: Oxidoreductase
Chromosomal Location of Human Ortholog: 15 E1|15 37.1 cM
Cellular Component: cytoplasm; cytosol; endosome; endosome membrane; membrane; NADPH oxidase complex; trans-Golgi network
Molecular Function: ARF guanyl-nucleotide exchange factor activity; lipid binding; phosphatidylinositol 3-phosphate binding; phosphatidylinositol binding; protein binding; superoxide-generating NADPH oxidase activator activity
Biological Process: phagocytosis; respiratory burst; vesicle-mediated transport
Research Articles on NCF4
Similar Products
Product Notes
The NCF4 ncf4 (Catalog #AAA2026214) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Neutrophil Cytosolic Factor 4 (NCF4) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophil Cytosolic Factor 4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-5ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NCF4 ncf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-AYM KNLLSLP VCVLMDPDVR IFFYQSAYDA EQVPQALRRL RPRTRKIKGV SPQGAIMDRM EAPRAEALFD FTGNSKLELS FKAGDVIFLL SKINKDWLEG TSQGATGIFP GSFVKILKDF PEDEDTTNWL RCYFYEDTGK TIKDIAVEED LSSTPLFKDL LALMRREFQR EDIALSYQDA EGDLVRLLSD EDVGLMVKQA. It is sometimes possible for the material contained within the vial of "Neutrophil Cytosolic Factor 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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