Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Neutrophil Cytosolic Factor 1 Polyclonal Antibody | anti-NCF1 antibody
Neutrophil Cytosolic Factor 1 (NCF1) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:HumanTissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Uniprot Description
p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.
Protein type: EC 1.-.-.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: cell soma; cytosol; dendrite; extrinsic component of membrane; Golgi apparatus; NADPH oxidase complex; plasma membrane; podosome; rough endoplasmic reticulum
Molecular Function: electron transfer activity; phosphatidylinositol binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activator activity; superoxide-generating NADPH oxidase activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; apoptosis; cell redox homeostasis; cellular defense response; cellular response to cadmium ion; cellular response to oxidative stress; cellular response to reactive oxygen species; innate immune response; podosome assembly; positive regulation of epidermal growth factor-activated receptor activity; positive regulation of JNK cascade; positive regulation of p38MAPK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription, DNA-templated; protein targeting to membrane; respiratory burst; superoxide anion generation; superoxide metabolic process; vascular endothelial growth factor receptor signaling pathway
Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I
Research Articles on NCF1
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Product Notes
The NCF1 ncf1 (Catalog #AAA2032502) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neutrophil Cytosolic Factor 1 (NCF1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophil Cytosolic Factor 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NCF1 ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neutrophil Cytosolic Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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