Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3) or DYKDDDDK-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Rabbit anti-Human Aurora C Polyclonal Antibody | anti-AURKC antibody
Aurora C, NT (G11) (Aurora/IPL1/Eg2 Protein 2, Aurora/IPL1-related Kinase 3, Serine/Threonine Protein Kinase 13, Aurora-related Kinase 3, ARK-3, Aurora Kinase C, Serine/Threonine-protein Kinase Aurora-C, AURKC, AIE2, AIK3, ARK3, STK13) (Azide free) (HRP)
Gene Names
AURKC; AIE2; AIK3; ARK3; AurC; SPGF5; STK13; aurora-C
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
Aurora C; Polyclonal Antibody; NT (G11) (Aurora/IPL1/Eg2 Protein 2; Aurora/IPL1-related Kinase 3; Serine/Threonine Protein Kinase 13; Aurora-related Kinase 3; ARK-3; Aurora Kinase C; Serine/Threonine-protein Kinase Aurora-C; AURKC; AIE2; AIK3; ARK3; STK13) (Azide free) (HRP); anti-AURKC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Aurora-C.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-AURKC antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human Aurora-C (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3) or DYKDDDDK-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Western Blot (WB)
(The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing DYKDDDDK tag (lane 1), DYKDDDDK-tagged Aurora A (lane 2), DYKDDDDK-tagged Aurora B (lane 3) or DYKDDDDK-tagged Aurora C (lane 4). In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).)
Western Blot (WB)
(Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).)
Western Blot (WB)
(Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).)
Product Categories/Family for anti-AURKC antibody
References
1. Kimura, M., et al., J. Biol. Chem. 274(11):7334-7340 (1999). 2. Tseng, T.C., et al., DNA Cell Biol. 17(10):823-833 (1998). 3. Bernard, M., et al., Genomics 53(3):406-409 (1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
309
NCBI Official Full Name
aurora kinase C isoform 1
NCBI Official Synonym Full Names
aurora kinase C
NCBI Official Symbol
AURKC
NCBI Official Synonym Symbols
AIE2; AIK3; ARK3; AurC; SPGF5; STK13; aurora-C
NCBI Protein Information
aurora kinase C; ARK-3; aurora 3; aurora-related kinase 3; aurora/IPL1/EG2 protein 2; aurora/IPL1-related kinase 3; serine/threonine-protein kinase 13; serine/threonine-protein kinase aurora-C; serine/threonine kinase 13 (aurora/IPL1-like)
UniProt Protein Name
Aurora kinase C
Protein Family
UniProt Gene Name
AURKC
UniProt Synonym Gene Names
AIE2; AIK3; AIRK3; ARK3; STK13; ARK-3
UniProt Entry Name
AURKC_HUMAN
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Product Notes
The AURKC aurkc (Catalog #AAA6464556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aurora C, NT (G11) (Aurora/IPL1/Eg2 Protein 2, Aurora/IPL1-related Kinase 3, Serine/Threonine Protein Kinase 13, Aurora-related Kinase 3, ARK-3, Aurora Kinase C, Serine/Threonine-protein Kinase Aurora-C, AURKC, AIE2, AIK3, ARK3, STK13) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aurora C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AURKC aurkc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aurora C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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