Rabbit anti-Human SALL4 Polyclonal Antibody | anti-SALL4 antibody

SALL4, CT (Sal-like Protein 4, Zinc Finger Protein SALL4) (Azide free) (HRP)

Cat. Num. AAA6498418

• Gene Names: SALL4; DRRS; HSAL4; ZNF797; dJ1112F19.1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: SALL4; Polyclonal Antibody; CT (Sal-like Protein 4; Zinc Finger Protein SALL4) (Azide free) (HRP); anti-SALL4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SALL4.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SALL4 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1009-1039 in the C-terminal region of human SALL4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS624296. SALL4 (arrow) was detected using MBS624296.)

Related Product Information for anti-SALL4 antibody

Sall4 is a probable transcription factor. Defects in Sall4 are the cause of Okihiro syndrome; also known as Duane radial ray syndrome (DRRS). It is a disorder characterized by the association of forearm malformations with Duane retraction syndrome. Sall4 is also involved in forelimb and heart development in mice.

Product Categories/Family for anti-SALL4 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Borozdin, W., Hum. Mutat. 28(8), 830 (2007). 2. Yang, J., Proc. Natl. Acad. Sci. USA 104(25), 10494-10499 (2007). 3. Paradisi, I., Am. J. Med. Genet. A 143(4), 326-332 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 10047144
• NCBI GeneID: 57167
• NCBI Accession #: NP_065169
• NCBI GenBank Nucleotide #: NM_020436
• UniProt Accession #: Q9UJQ4; Q540H3; Q6Y8G6; A2A2D8
• Molecular Weight: 65,708 Da
• NCBI Official Full Name: sal-like protein 4
• NCBI Official Synonym Full Names: spalt-like transcription factor 4
• NCBI Official Symbol: SALL4
• NCBI Official Synonym Symbols: DRRS; HSAL4; ZNF797; dJ1112F19.1
• NCBI Protein Information: sal-like protein 4; zinc finger protein 797; zinc finger protein SALL4
• UniProt Protein Name: Sal-like protein 4
• Protein Family: Sal-like protein
• UniProt Gene Name: SALL4
• UniProt Synonym Gene Names: ZNF797
• UniProt Entry Name: SALL4_HUMAN

Uniprot Description

SALL4: a transcription factor and oncofetal protein expressed embryonically and in the testis. Plays a key role in maintenance and self-renewal of embryonic stem cells. Interacts with NANOG and OCT4. Ectopically expressed in acute B-cell lymphoblastic leukemia (B-ALL) and myeloid leukemia (AML), playing a role in the survival of B-ALL cells and in myeloid leukemogenesis. Aberrantly expressed in multiple carcinomas including human gastric carcinoma and hepatocellular carcinoma. Maintains the stem cell qualities of EpCAM-positive hepatocellular carcinoma cells. Expressed in primary human endometrial cancer samples but not normal or hyperplastic endometrial cells. Its expression in carcinomas positively correlates with metastatic potential and poor patient survival. Specifically binds the c-Myc promoter region in endometrial cancer cells, apparently increasing c-Myc expression. Belongs to the sal C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: nucleoplasm; heterochromatin; protein complex; intracellular membrane-bound organelle; cytoplasm

Molecular Function: DNA binding; metal ion binding

Biological Process: transcription, DNA-dependent; somatic stem cell maintenance; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation; embryonic limb morphogenesis

Disease: Ivic Syndrome; Duane-radial Ray Syndrome