Rabbit Neuropilin-1 Polyclonal Antibody | anti-NRP1 antibody

Anti-Neuropilin-1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103512

• Gene Names: Nrp1; Nrp; NP-1; Npn1; NPN-1; C530029I03
• Reactivity: Mouse, Rat, Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Neuropilin-1; Polyclonal Antibody; Anti-Neuropilin-1 Antibody; Rabbit Polyclonal; Neuropilin-1/NRP1 Antibody; Rabbit PAb; Antigen Affinity Purified; neuropilin 1; Anti-C530029I03 Antibody; Anti-NP-1 Antibody; Anti-NPN-1 Antibody; Anti-Npn1 Antibody; Anti-Nrp Antibody; anti-NRP1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat, Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse Neuropilin-1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NRP1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Mouse Neuropilin-1/NRP1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse Neuropilin-1/NRP1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in rat brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in rat liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining NRP1 in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-NRP1 antibody

Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

References

Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.

NCBI and Uniprot Product Information

• NCBI GI #: 244792700
• NCBI GeneID: 18186
• NCBI Accession #: NP_032763.2
• NCBI GenBank Nucleotide #: NM_008737.2
• UniProt Accession #: P97333; Q6PAR3
• Molecular Weight: 103,000 Da
• NCBI Official Full Name: neuropilin-1
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: Nrp1
• NCBI Official Synonym Symbols: Nrp; NP-1; Npn1; NPN-1; C530029I03
• NCBI Protein Information: neuropilin-1
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: Nrp1
• UniProt Synonym Gene Names: Nrp

Uniprot Description

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 8 E2|8 75.78 cM

Cellular Component: axon; cell soma; cell surface; cytosol; early endosome; extracellular space; focal adhesion; growth cone; neurofilament; plasma membrane

Molecular Function: growth factor binding; protein binding; semaphorin receptor activity; vascular endothelial growth factor receptor activity

Biological Process: angiogenesis; artery morphogenesis; axon extension involved in axon guidance; axon guidance; axonal fasciculation; branchiomotor neuron axon guidance; cell migration; cell migration during sprouting angiogenesis; dendrite development; facial nerve structural organization; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; heart development; hepatocyte growth factor receptor signaling pathway; motor axon guidance; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; negative regulation of neuron apoptosis; nerve development; neuron development; neuron migration; patterning of blood vessels; platelet-derived growth factor receptor signaling pathway; positive chemotaxis; positive regulation of axon extension involved in axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; regulation of axon extension involved in axon guidance; retinal ganglion cell axon guidance; sprouting angiogenesis; sympathetic nervous system development; trigeminal nerve morphogenesis; trigeminal nerve structural organization; vascular endothelial growth factor receptor signaling pathway; vestibulocochlear nerve structural organization