Mouse Neuropilin-1 ELISA Kit | NRP1 elisa kit

Mouse Neuropilin-1 PicoKine ELISA Kit

Cat. Num. AAA177111

• Gene Names: Nrp1; Nrp; NP-1; Npn1; NPN-1; C530029I03
• Reactivity: Mouse
• Synonyms: Neuropilin-1; Mouse Neuropilin-1 PicoKine ELISA Kit; neuropilin 1; A5 protein; BDCA4; BLOOD DENDRITIC CELL ANTIGEN 4; CD 304; CD304; DKFZp686A03134; DKFZp781F1414; Neuropilin1; Neuropilin1 transmembrane receptor; NP1; NPN1; NRP 1; NRP; NRP1; NRP1_HUMAN; Vascular endothelial cell growth factor 165 receptor; VEGF165R; NRP1 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Neuropilin-1
• Sequence Length: 923

• Samples: Cell culture supernates, serum, and plasma(heparin, EDTA).
• Assay Type: Sandwich
• Detection Range: 780pg/ml-50000pg/ml
• Sensitivity: <20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse Neuropilin-1 PicoKine ELISA Kit standard curve )

Related Product Information for NRP1 elisa kit

Principle of the Assay: The Mouse Neuropilin-1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from rat specific for Neuropilin-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: F22-P856) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuropilin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Neuropilin-1 amount of sample captured in plate.

Background: NRP1(Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.

NCBI and Uniprot Product Information

• NCBI GI #: 341941196
• NCBI GeneID: 18186
• UniProt Accession #: P97333; Q6PAR3
• Molecular Weight: 103,000 Da
• NCBI Official Full Name: Neuropilin-1
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: Nrp1
• NCBI Official Synonym Symbols: Nrp; NP-1; Npn1; NPN-1; C530029I03
• NCBI Protein Information: neuropilin-1; A5 protein; transmembrane receptor; Neuropilin-1 precursor (A5 protein)
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: Nrp1
• UniProt Synonym Gene Names: Nrp
• UniProt Entry Name: NRP1_MOUSE

Uniprot Description

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Cellular Component: extracellular space; focal adhesion; cell surface; growth cone; membrane; cell soma; axon; early endosome; plasma membrane; integral to membrane; neurofilament; cytosol

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; metal ion binding; growth factor binding

Biological Process: facial nerve structural organization; axon guidance; peptidyl-tyrosine phosphorylation; heart development; multicellular organismal development; neuron migration; motor axon guidance; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; negative regulation of axon extension; negative regulation of axon extension involved in axon guidance; trigeminal nerve morphogenesis; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; sympathetic nervous system development; positive chemotaxis; branchiomotor neuron axon guidance; dendrite development; negative regulation of neuron apoptosis; angiogenesis; cell differentiation; nervous system development; trigeminal nerve structural organization; cell migration; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; patterning of blood vessels; axon extension involved in axon guidance; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; otic placode formation; regulation of axon extension involved in axon guidance; positive regulation of axon extension involved in axon guidance; neuron development; artery morphogenesis; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance