Western Blot (WB)
((0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Neuropeptide Y receptor Y5 Polyclonal Antibody | anti-NPY5R antibody
Goat anti-Neuropeptide Y receptor Y5 Antibody
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Western blot: Approx 50kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7kDa according to NP_006165.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on CHO-K1 cells: PMID: 30503694. This antibody has been successfully used in WB on Human: PMID: 28413811 and 20332211.
Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6µg/ml.
Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694.
Western Blot (WB)
((0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a receptor for neuropeptide Y and peptide YY. The encoded protein appears to be involved in regulating food intake, with defects in this gene being associated with eating disorders. Also, the encoded protein is involved in a pathway that protects neuroblastoma cells from chemotherapy-induced cell death, providing a possible therapeutic target against neuroblastoma. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2015]
Uniprot Description
NPY5R: Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 4q31-q32
Cellular Component: cytoplasm; integral to plasma membrane; neuron projection; plasma membrane
Molecular Function: neuropeptide Y receptor activity; pancreatic polypeptide receptor activity; peptide YY receptor activity
Biological Process: cell surface receptor linked signal transduction; eating behavior; feeding behavior; G-protein coupled receptor protein signaling pathway; generation of ovulation cycle rhythm; negative regulation of apoptosis; negative regulation of glutamate secretion; negative regulation of synaptic transmission, GABAergic; neuropeptide signaling pathway; positive regulation of acute inflammatory response; positive regulation of smooth muscle cell proliferation; synaptic transmission
Research Articles on NPY5R
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Product Notes
The NPY5R npy5r (Catalog #AAA420766) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Neuropeptide Y receptor Y5 Antibody reacts with Tested: Human Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Neuropeptide Y receptor Y5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kDa band observed in Human Brain, Breast and Duodenum lysates (calculated MW of 50.7kDa according to NP_006165.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on CHO-K1 cells: PMID: 30503694. This antibody has been successfully used in WB on Human: PMID: 28413811 and 20332211. Immunohistochemistry: In paraffin embedded Human Intestine shows staining of ganglion cells. Recommended concentration, 4-6µg/ml. Immunoprecipitation: Customer feedback showed specific results when this product was used in IP. This antibody has been successfully used in IP: PMID: 30503694. Researchers should empirically determine the suitability of the NPY5R npy5r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GLSNKENRLE EN. It is sometimes possible for the material contained within the vial of "Neuropeptide Y receptor Y5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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