Western Blot (WB)
((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat neuronal pentraxin 1 Polyclonal Antibody | anti-NPTX1 antibody
Goat anti-neuronal pentraxin 1 Antibody
Gene Names
NPTX1; NP1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
neuronal pentraxin 1; Polyclonal Antibody; Goat anti-neuronal pentraxin 1 Antibody; NPTX1; neuronal pentraxin I; NP1; NP-I; neuronal pentraxin-1; NPTX1 antibody; neuronal pentraxin I antibody; NP1 antibody; NP-I antibody; neuronal pentraxin-1 antibody; anti-NPTX1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
ERVKIETALTSLHQR
Sequence Length
432
Applicable Applications for anti-NPTX1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3ug/ml. Additional bands of unknown identity was also consistently observed at 38 and 14kDa.
Western Blot: Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3ug/ml. Additional bands of unknown identity was also consistently observed at 38 and 14kDa.
Immunogen
Peptide with sequence C-ERVKIETALTSLHQR, from the internal region of the protein sequence according to NP_002513.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,122 Da
NCBI Official Full Name
neuronal pentraxin-1
NCBI Official Synonym Full Names
neuronal pentraxin 1
NCBI Official Symbol
NPTX1
NCBI Official Synonym Symbols
NP1
NCBI Protein Information
neuronal pentraxin-1
UniProt Protein Name
Neuronal pentraxin-1
Protein Family
UniProt Gene Name
NPTX1
UniProt Synonym Gene Names
NP1; NP-I
UniProt Entry Name
NPTX1_HUMAN
NCBI Description
NPTX1 is a member of the neuronal pentraxin gene family. Neuronal pentraxin 1 is similar to the rat NP1 gene which encodes a binding protein for the snake venom toxin taipoxin. Human NPTX1 mRNA is exclusively localized to the nervous system. [provided by RefSeq, Jul 2008]
Uniprot Description
NPTX1: May mediate uptake of degraded synaptic material which could play an important role in synaptic remodeling. Can mediate the neuronal and glial uptake of the snake venom toxin taipoxin.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: transport vesicle
Molecular Function: metal ion binding
Biological Process: central nervous system development; synaptic transmission; transport
Research Articles on NPTX1
Similar Products
Product Notes
The NPTX1 nptx1 (Catalog #AAA423400) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-neuronal pentraxin 1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's neuronal pentraxin 1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 50kDa band observed in Human Brain (Amygdala and Frontal Cortex) lysates (calculated MW of 47.1kDa according to NP_002513.2). Recommended concentration: 1-3ug/ml. Additional bands of unknown identity was also consistently observed at 38 and 14kDa. Researchers should empirically determine the suitability of the NPTX1 nptx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ERVKIETALT SLHQR. It is sometimes possible for the material contained within the vial of "neuronal pentraxin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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