Goat Neuroligin 3 Polyclonal Antibody | anti-NLGN3 antibody

Goat anti-Neuroligin 3 Antibody

Cat. Num. AAA420494

• Gene Names: NLGN3; HNL3
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Neuroligin 3; Polyclonal Antibody; Goat anti-Neuroligin 3 Antibody; neuroligin 3; KIAA1480; ASPGX1; AUTSX1; HNL3; NLGN3; neuroligin 3 antibody; KIAA1480 antibody; ASPGX1 antibody; AUTSX1 antibody; HNL3 antibody; NLGN3 antibody; anti-NLGN3 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: RKDKRRQEPLRQPS
• Sequence Length: 828

• Applicable Applications for anti-NLGN3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Approx.85-90kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 91.6kDa according to NP_061850.2). In transfected HEK293 transiently expressing Neuregulin3 a band of approx.98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 05-0.2ug/ml.
• Immunogen: Peptide with sequence C-RKDKRRQEPLRQPS, from the internal region of the protein sequence according to NP_061850.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing Neuregulin3 (RC207955) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

• NCBI GI #: 51593088
• NCBI GeneID: 54413
• NCBI Accession #: NP_061850.2
• NCBI GenBank Nucleotide #: NM_018977.3
• Molecular Weight: 89,538 Da
• NCBI Official Full Name: neuroligin-3 isoform 2
• NCBI Official Synonym Full Names: neuroligin 3
• NCBI Official Symbol: NLGN3
• NCBI Official Synonym Symbols: HNL3
• NCBI Protein Information: neuroligin-3
• UniProt Protein Name: Neuroligin-3
• Protein Family: Neuroligin
• UniProt Gene Name: NLGN3
• UniProt Synonym Gene Names: KIAA1480; NL3
• UniProt Entry Name: NLGN3_HUMAN

NCBI Description

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

NLGN3: Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system. Defects in NLGN3 may be the cause of susceptibility to autism X-linked type 1 (AUTSX1). AUTSX1 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Defects in NLGN3 may be the cause of susceptibility to X- linked Asperger syndrome 1 (ASPGX1). ASPGX1 is considered to be a form of childhood autism. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: cell junction; cell soma; cell surface; dendrite; endocytic vesicle; excitatory synapse; extracellular space; integral to plasma membrane; synapse

Molecular Function: cell adhesion molecule binding; neurexin binding; protein binding; receptor activity

Biological Process: adult behavior; axon extension; learning; neurological control of breathing; neuron adhesion; oligodendrocyte differentiation; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; receptor-mediated endocytosis; regulation of excitatory postsynaptic membrane potential; regulation of inhibitory postsynaptic membrane potential; regulation of synaptic transmission; rhythmic synaptic transmission; social behavior; synapse organization and biogenesis; synaptogenesis; visual learning

Disease: Asperger Syndrome, X-linked, Susceptibility To, 1; Autism, Susceptibility To, X-linked 1