Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F antibody at 1:200 dilution.)
Rabbit hnRNP F Polyclonal Antibody | anti-hnRNP F antibody
hnRNP F antibody
Gene Names
Hnrnpf; Hnrpf; AA407306; 4833420I20Rik
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
hnRNP F antibody was purified by antigen-affinity chromatography
Synonyms
hnRNP F; Polyclonal Antibody; hnRNP F antibody; Polyclonal hnRNP F; Anti-hnRNP F; HNRPF; HnRNP F protein; MGC110997; OK/SW-cl.23; mcs94-1; HNRNPF; nucleolin-like protein mcs94-1; heterogeneous nuclear ribonucleoprotein F; anti-hnRNP F antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
hnRNP F antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.72 mg/ml (varies by lot)
Sequence Length
415
Applicable Applications for anti-hnRNP F antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IF:1:100-1:200
IHC-P: 1:100-1:250
ICC:1:100-1:200
WB: 1:500-1:3000
IHC-P: 1:100-1:250
ICC:1:100-1:200
WB: 1:500-1:3000
Biological Significance
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F antibody at 1:200 dilution.)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 10 % SDS PAGE gel and hnRNP F antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 10 % SDS PAGE gel and hnRNP F antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 Xenograft using hnRNP F antibody at a dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded A549 Xenograft using hnRNP F antibody at a dilution of 1:100)
Related Product Information for anti-hnRNP F antibody
Affinity purified Rabbit polyclonal hnRNP F antibody
Product Categories/Family for anti-hnRNP F antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46 kDa
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein F
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein F
NCBI Official Symbol
Hnrnpf
NCBI Official Synonym Symbols
Hnrpf; AA407306; 4833420I20Rik
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein F
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein F
UniProt Gene Name
Hnrnpf
UniProt Synonym Gene Names
Hnrpf; hnRNP F
UniProt Entry Name
HNRPF_MOUSE
Uniprot Description
hnRNP F: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.
Protein type: RNA-binding; Spliceosome; RNA splicing
Cellular Component: spliceosome; membrane; ribonucleoprotein complex; nucleus
Molecular Function: single-stranded RNA binding; nucleic acid binding; RNA binding; TATA-binding protein binding; nucleotide binding
Biological Process: regulation of RNA splicing; RNA splicing; mRNA processing
Research Articles on hnRNP F
Similar Products
Product Notes
The hnRNP F hnrnpf (Catalog #AAA835647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's hnRNP F can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IF:1:100-1:200 IHC-P: 1:100-1:250 ICC:1:100-1:200 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the hnRNP F hnrnpf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hnRNP F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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