Rabbit Neurofilament H, Extreme, Polyclonal Antibody
Neurofilament H, Extreme, (NF_H)
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Applications
Western Blot, Immunohistochemistry
Purity
SerumNeat Serum
Synonyms
Neurofilament H; Extreme; ; Polyclonal Antibody; (NF_H); Anti -Neurofilament H; anti-Neurofilament H; antibody
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Strong and specific staining for NF-H with no crossreactivity for NF-M or NF-L. Reacts with NF-H in human, mouse, rat, bovine and porcine, but not with avian NF-H
Purity/Purification
Serum
Neat Serum
Neat Serum
Form/Format
Supplied as a liquid containing 0.02% sodium azide
Applicable Applications for anti-Neurofilament H, Extreme, antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry, Immunocytochemistry.
Dilution: Immunohistochemistry: 1:200
Optimal dilutions to be determined by researcher.
Dilution: Immunohistochemistry: 1:200
Optimal dilutions to be determined by researcher.
Immunogen
Recombinant fusion protein containing the extreme C-terminus of rat NF-H, prepared from E. coli inclusion bodies by dissolution in urea followed by DEAE-cellulose ion exchange chromatography. Immunogen does not include the lysine-serine-proline multiphosphorylation repeat and stains neurofilaments irrespective of the level of phosphorylation
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Neurofilament H, Extreme, antibody
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160 and 200kD) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70kD NF-L protein can self-assemble into a filamentous structure. The 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung express neurofilaments.
Product Categories/Family for anti-Neurofilament H, Extreme, antibody
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Product Notes
The Neurofilament H, Extreme, (Catalog #AAA618624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament H, Extreme, (NF_H) reacts with Bovine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament H, Extreme, can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry, Immunocytochemistry. Dilution: Immunohistochemistry: 1:200 Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Neurofilament H, Extreme, for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament H, Extreme,, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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